PMID- 23453270
OWN - NLM
STAT- MEDLINE
DCOM- 20141021
LR  - 20151119
IS  - 1878-4119 (Electronic)
IS  - 1010-5182 (Linking)
VI  - 41
IP  - 8
DP  - 2013 Dec
TI  - Amplification of the PPFIA1 gene region on 11q13 in oral squamous cell carcinomas 
      (OSCC).
PG  - 845-9
LID - S1010-5182(13)00056-5 [pii]
LID - 10.1016/j.jcms.2013.01.040 [doi]
AB  - INTRODUCTION: Analyzing chromosomal amplifications delivers valuable information 
      for identification of oncogenes. For carcinomas of the oral cavity only few genes 
      have been identified in amplified regions. The aim of this study was to search 
      genes in amplified regions as possible biomarkers and targets for novel 
      therapies. MATERIAL AND METHODS: DNA from 10 carcinomas of the floor of the oral 
      cavity was examined using a 500K Array GeneChip (Affymetrix 6.0) to detect 
      chromosomal losses, gains or amplifications. Suspicious alterations were 
      validated on tissue microarrays using fluorescence in situ hybridization (FISH) 
      with respective probes. RESULTS: FISH-validation on tissue arrays confirmed 
      PPFIA1 amplifications as one of the most frequent events (32.6%). High (10-20 
      signals) and low (<10 signals) amplification of PPFIA1 was found in 10.9% (5/46) 
      and 21.7% (10/46) tumours, respectively. Fine mapping with overlapping FISH 
      probes showed co-amplification of PPFIA1 and the Cyclin D1 gene which are 
      approximately 600 kb apart from each other, likely in the same amplicon. 
      DISCUSSION: PPFIA1 was frequently co-amplified with the Cyclin D1 gene in oral 
      carcinomas and could present a biomarker as well as a novel target for specific 
      gene therapy. Further studies are necessary to investigate the role of PPFIA1 in 
      development and pathogenesis of oral carcinomas.
CI  - Copyright (c) 2013 European Association for Cranio-Maxillo-Facial Surgery. 
      Published by Elsevier Ltd. All rights reserved.
FAU - Blessmann, Marco
AU  - Blessmann M
AD  - Department of Oral and Maxillofacial Surgery, University Medical Center 
      Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
FAU - Al-Dam, Ahmed
AU  - Al-Dam A
FAU - Hanken, Henning
AU  - Hanken H
FAU - Assaf, Alexandre T
AU  - Assaf AT
FAU - Riecke, Bjorn
AU  - Riecke B
FAU - Klatt, Jan
AU  - Klatt J
FAU - Simon, Ronald
AU  - Simon R
FAU - Sauter, Guido
AU  - Sauter G
FAU - Heiland, Max
AU  - Heiland M
FAU - Kluwe, Lan
AU  - Kluwe L
FAU - Grobe, Alexander
AU  - Grobe A
LA  - eng
PT  - Journal Article
DEP - 20130226
PL  - Scotland
TA  - J Craniomaxillofac Surg
JT  - Journal of cranio-maxillo-facial surgery : official publication of the European 
      Association for Cranio-Maxillo-Facial Surgery
JID - 8704309
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CCND1 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (PPFIA1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics
MH  - Biomarkers, Tumor/analysis
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Adhesion Molecules/*genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Cyclin D1/genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/genetics
MH  - Male
MH  - Mouth Neoplasms/*genetics
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pharyngeal Neoplasms/genetics
MH  - Tissue Array Analysis
OTO - NOTNLM
OT  - Amplification
OT  - Biomarker
OT  - Cyclin D1
OT  - Oral squamous cell carcinoma
OT  - PPFIA1
OT  - Target therapy
EDAT- 2013/03/05 06:00
MHDA- 2014/10/22 06:00
CRDT- 2013/03/05 06:00
PHST- 2012/09/26 00:00 [received]
PHST- 2013/01/05 00:00 [revised]
PHST- 2013/01/07 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2014/10/22 06:00 [medline]
AID - S1010-5182(13)00056-5 [pii]
AID - 10.1016/j.jcms.2013.01.040 [doi]
PST - ppublish
SO  - J Craniomaxillofac Surg. 2013 Dec;41(8):845-9. doi: 10.1016/j.jcms.2013.01.040. 
      Epub 2013 Feb 26.