PMID- 23453491
OWN - NLM
STAT- MEDLINE
DCOM- 20140314
LR  - 20161125
IS  - 1532-3129 (Electronic)
IS  - 0021-9975 (Linking)
VI  - 149
IP  - 2-3
DP  - 2013 Aug-Oct
TI  - Immunohistochemical characterization of type II pneumocyte proliferation after 
      challenge with type I porcine reproductive and respiratory syndrome virus.
PG  - 322-30
LID - S0021-9975(12)00439-2 [pii]
LID - 10.1016/j.jcpa.2012.12.006 [doi]
AB  - The aim of this study was to characterize histologically and 
      immunohistochemically the lung lesions developing in growing pigs, 10 and 21 days 
      after experimental challenge with a field strain of porcine reproductive and 
      respiratory syndrome virus (PRRSV). Lung lesions were scored for (1) pneumocyte 
      hypertrophy and hyperplasia, (2) septal mononuclear infiltration, (3) 
      intra-alveolar necrotic debris, (4) intra-alveolar inflammatory cell accumulation 
      and (5) perivascular inflammatory cell accumulation. Immunohistochemistry was 
      performed using antibodies specific for cytokeratin, Ki67, thyroid transcription 
      factor (TTF)-1, the myelomonocytic marker MAC387 and PRRSV. Anti-TTF-1 identified 
      type II pneumocytes and there was marked proliferation of these cells compared 
      with control lung (P <0.05). Anti-cytokeratin labelled type I and II pneumocytes 
      as well as bronchial epithelial cells; however, this labelling was not suitable 
      for cell counting purposes. There was a correlation between lesion severity and 
      the number of cells expressing Ki67 (P <0.05).
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Balka, G
AU  - Balka G
AD  - Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary 
      Science, Szent Istvan University, Budapest, Hungary. balka.gyula@aotk.szie.hu
FAU - Ladinig, A
AU  - Ladinig A
FAU - Ritzmann, M
AU  - Ritzmann M
FAU - Saalmuller, A
AU  - Saalmuller A
FAU - Gerner, W
AU  - Gerner W
FAU - Kaser, T
AU  - Kaser T
FAU - Jakab, C
AU  - Jakab C
FAU - Rusvai, M
AU  - Rusvai M
FAU - Weissenbock, H
AU  - Weissenbock H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130228
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
SB  - IM
MH  - Alveolar Epithelial Cells/*metabolism/pathology
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunohistochemistry
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/pathology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
OTO - NOTNLM
OT  - PRRSV
OT  - TTF-1
OT  - pig
OT  - type II pneumocyte
EDAT- 2013/03/05 06:00
MHDA- 2014/03/15 06:00
CRDT- 2013/03/05 06:00
PHST- 2012/09/15 00:00 [received]
PHST- 2012/11/15 00:00 [revised]
PHST- 2012/12/19 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2014/03/15 06:00 [medline]
AID - S0021-9975(12)00439-2 [pii]
AID - 10.1016/j.jcpa.2012.12.006 [doi]
PST - ppublish
SO  - J Comp Pathol. 2013 Aug-Oct;149(2-3):322-30. doi: 10.1016/j.jcpa.2012.12.006. 
      Epub 2013 Feb 28.