PMID- 23453581
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR  - 20211021
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 439
IP  - 1
DP  - 2013 Apr 25
TI  - Replacement of the heterologous 5' untranslated region allows preservation of the 
      fully functional activities of type 2 porcine reproductive and respiratory 
      syndrome virus.
PG  - 1-12
LID - S0042-6822(12)00641-1 [pii]
LID - 10.1016/j.virol.2012.12.013 [doi]
AB  - The 5' untranslated region (UTR) is believed to be vital for the replication of 
      porcine reproductive and respiratory syndrome virus (PRRSV), yet its functional 
      mechanism remains largely unknown. In this study, to define the cis-acting 
      elements for viral replication and infectivity, The 5' UTR swapping chimeric 
      clones pTLV8 and pSHSP5 were constructed based on two different genotypes 
      full-length infectious cDNA clone pAPRRS and pSHE backbones. Between them, vTLV8 
      could be rescued from pTLV8 and had similar virological properties to vAPRRS, 
      including phenotypic characteristic and RNA synthesis level. However, pSHSP5 
      exhibited no evidence of infectivity. Taken together, the results presented here 
      demonstrate that only the 5' UTR of type 1 PRRSV did not affect the infectivity 
      and replication of type 2 PRRSV in vitro. The 5' UTR of type 2 PRRSV could be 
      functionally replaced by its counterpart from type 1.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Yao, Huochun
AU  - Yao H
FAU - Lu, Jiaqi
AU  - Lu J
FAU - Wei, Zuzhang
AU  - Wei Z
FAU - Zheng, Haihong
AU  - Zheng H
FAU - Zhuang, Jinshan
AU  - Zhuang J
FAU - Tong, Guangzhi
AU  - Tong G
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130301
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/genetics/growth & 
      development/*physiology
MH  - RNA, Viral/*genetics
MH  - Recombination, Genetic
MH  - Sequence Alignment
MH  - Swine
MH  - Viral Load
MH  - Viral Plaque Assay
MH  - *Virus Replication
PMC - PMC7111940
EDAT- 2013/03/05 06:00
MHDA- 2013/05/08 06:00
PMCR- 2013/03/01
CRDT- 2013/03/05 06:00
PHST- 2012/09/30 00:00 [received]
PHST- 2012/12/20 00:00 [revised]
PHST- 2012/12/25 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - S0042-6822(12)00641-1 [pii]
AID - 10.1016/j.virol.2012.12.013 [doi]
PST - ppublish
SO  - Virology. 2013 Apr 25;439(1):1-12. doi: 10.1016/j.virol.2012.12.013. Epub 2013 
      Mar 1.