PMID- 23454340
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 45
DP  - 2013 Jul 15
TI  - Electrochemical evaluation of DNA methylation level based on the stoichiometric 
      relationship between purine and pyrimidine bases.
PG  - 34-9
LID - S0956-5663(13)00068-7 [pii]
LID - 10.1016/j.bios.2013.01.057 [doi]
AB  - An efficient electrochemical approach for the evaluation of DNA methylation level 
      was proposed according to the oxidation signal of DNA bases at an overoxidized 
      polypyrrole (PPyox) directed multiwalled carbon nanotubes (MWNTs) film modified 
      glassy carbon electrode (GCE). The PPyox/MWNTs/GCE exhibited remarkable 
      electrocatalytic activities towards the oxidation of DNA bases due to the 
      advantages of wide potential window, large effective surface area, and excellent 
      antifouling property. As a result, all purine and pyrimidine bases of guanine 
      (G), adenine (A), thymine (T), cytosine (C) and 5-methylcytosine (5-mC) exhibited 
      well identified oxidation peaks at the PPyox/MWNTs/GCE. The direct potential 
      resolution between 5-mC and C was obtained to be 180 mV, which was large enough 
      for their signal recognition and accurate detection in mixture. In particular, 
      the signal interference from T, a great challenge in exploring DNA methylation, 
      was successfully eliminated by an innovative strategy, which was developed based 
      on the stoichiometric relationship between purine and pyrimidine bases in DNA 
      molecular structure. The proposed method was effectively applied to the rapid 
      detection of DNA methylation status in real sample within 45 min with 
      satisfactory results.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wang, Po
AU  - Wang P
AD  - Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, 
      School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou 
      221116, PR China. wangpo@jsnu.edu.cn
FAU - Chen, Hanbin
AU  - Chen H
FAU - Tian, Jiuying
AU  - Tian J
FAU - Dai, Zong
AU  - Dai Z
FAU - Zou, Xiaoyong
AU  - Zou X
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130207
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Purines)
RN  - 0 (Pyrimidines)
RN  - 9007-49-2 (DNA)
RN  - K8CXK5Q32L (pyrimidine)
RN  - W60KTZ3IZY (purine)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - DNA/chemistry/*isolation & purification
MH  - DNA Methylation
MH  - Electrochemical Techniques
MH  - Humans
MH  - Nanotubes, Carbon/chemistry
MH  - Oxidation-Reduction
MH  - Purines/*isolation & purification
MH  - Pyrimidines/*isolation & purification
EDAT- 2013/03/05 06:00
MHDA- 2013/09/24 06:00
CRDT- 2013/03/05 06:00
PHST- 2012/10/29 00:00 [received]
PHST- 2013/01/17 00:00 [revised]
PHST- 2013/01/23 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
AID - S0956-5663(13)00068-7 [pii]
AID - 10.1016/j.bios.2013.01.057 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Jul 15;45:34-9. doi: 10.1016/j.bios.2013.01.057. Epub 
      2013 Feb 7.