PMID- 23455187
OWN - NLM
STAT- MEDLINE
DCOM- 20140610
LR  - 20161020
IS  - 1533-4058 (Electronic)
IS  - 1533-4058 (Linking)
VI  - 21
IP  - 6
DP  - 2013 Dec
TI  - One-day FISH approach for the high-speed determination of HER2 gene copy status 
      in breast carcinoma.
PG  - 567-71
LID - 10.1097/PAI.0b013e318288dcdc [doi]
AB  - Fluorescence in situ hybridization (FISH) is a commonly used method to detect 
      chromosomal aberrations, for example, to assess human epidermal growth factor 
      receptor 2 (HER2) gene status in breast carcinoma. The classical FISH approach 
      requires overnight incubation for proper hybridization result. Tissue morphologic 
      features are varying because of aggressive pretreatment and application of high 
      temperatures. To eliminate some of the methodological problems, a new 1-day FISH 
      method was recently introduced. The aim of our study was to evaluate the utility 
      of the Instant Quality FISH with the conventional FISH kit from the same provider 
      (Dako pharmDx) for determination of HER2 status. We performed in situ 
      hybridization on the same 40 invasive breast carcinoma samples with both probe 
      kits, and HER2/CEN17 and chromosome 17/cell nucleus ratios were calculated. FISH 
      signal stability was also tested by the reassessment of the slides after 2 months 
      storage. The accordance regarding HER2 gene amplification status between the 2 
      FISH kits tested was 100%. There was an excellent correlation between HER2/CEN17 
      ratios with a concordance correlation coefficient of 0.958 and correlation 
      coefficient (R) of 0.959. The 1-day HER2 Instant Quality FISH diagnostic kit 
      points with fast and stable reaction showing the same result in the diagnostic 
      practice when compared with the conventional overnight FISH method.
FAU - Hegyi, Katalin
AU  - Hegyi K
AD  - *Department of Pathology, Medical and Health Sciences Center, University of 
      Debrecen, Debrecen, Hungary daggerDako A/S, Glostrup, Denmark.
FAU - Lonborg, Charlotte
AU  - Lonborg C
FAU - Monus, Aniko
AU  - Monus A
FAU - Mehes, Gabor
AU  - Mehes G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Breast Neoplasms/*diagnosis/genetics/pathology
MH  - Carcinoma, Ductal, Breast/*diagnosis/genetics/pathology
MH  - Centromere/chemistry
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Reagent Kits, Diagnostic
MH  - Receptor, ErbB-2/*genetics
MH  - Time Factors
EDAT- 2013/03/05 06:00
MHDA- 2014/06/11 06:00
CRDT- 2013/03/05 06:00
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2014/06/11 06:00 [medline]
AID - 10.1097/PAI.0b013e318288dcdc [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2013 Dec;21(6):567-71. doi: 
      10.1097/PAI.0b013e318288dcdc.