PMID- 23455784
OWN - NLM
STAT- MEDLINE
DCOM- 20131118
LR  - 20191210
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 55
IP  - 4
DP  - 2012 Oct-Dec
TI  - Correlation between HER2 gene amplification and protein overexpression through 
      fluorescence in situ hybridization and immunohistochemistry in breast carcinoma 
      patients.
PG  - 481-4
LID - 10.4103/0377-4929.107785 [doi]
AB  - BACKGROUND: In India, the incidence of breast cancer has increased in the urban 
      population, with 1 in every 22 women diagnosed with breast cancer. It is 
      important to know the HER2/neu gene status for a better prognostication of these 
      patients. AIM: The aim of this study was to compare the efficacy of fluorescence 
      in situ hybridization (FISH) and immunohistochemistry (IHC) for determining 
      HER2/neu alteration in breast carcinoma. MATERIALS AND METHODS: A total of 188 
      histologically proven breast carcinoma cases between the years 2007 and 2011 were 
      retrospectively analyzed on the paraffin tissue sections by both IHC and FISH 
      techniques. FISH for HER2/neu gene amplification was performed on cases where the 
      IHC status was already known and the results were compared. RESULTS: A total of 
      64 (30%) patients were found to be amplified and the remaining 124 (65.9%) cases 
      were found to be unamplified through FISH. Patients observed with 3+ reading on 
      IHC were later confirmed as unamplified in 29.5% cases through FISH. CONCLUSION: 
      It has been confirmed with the present study that IHC is a prudent first-step 
      technique to screen tissue samples for HER2/neu gene status, but should be 
      supplemented with the FISH technique especially in equivocal cases.
FAU - Makroo, R N
AU  - Makroo RN
AD  - Transplant Immunology, Molecular Biology and Transfusion Medicine, Apollo 
      Hospitals, Sarita Vihar, Delhi Mathura Road, New Delhi, India.
FAU - Chowdhry, Mohit
AU  - Chowdhry M
FAU - Kumar, Manoj
AU  - Kumar M
FAU - Srivastava, Priyanka
AU  - Srivastava P
FAU - Tyagi, Richa
AU  - Tyagi R
FAU - Bhadauria, Preeti
AU  - Bhadauria P
FAU - Kaul, Sumaid
AU  - Kaul S
FAU - Sarin, Ramesh
AU  - Sarin R
FAU - Das, P K
AU  - Das PK
FAU - Dua, Harsh
AU  - Dua H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/*pathology
MH  - Carcinoma/*pathology
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization/*methods
MH  - India
MH  - Mass Screening/methods
MH  - Middle Aged
MH  - Pathology/*methods
MH  - Receptor, ErbB-2/*analysis/genetics
MH  - Retrospective Studies
EDAT- 2013/03/05 06:00
MHDA- 2013/11/19 06:00
CRDT- 2013/03/05 06:00
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/11/19 06:00 [medline]
AID - IndianJPatholMicrobiol_2012_55_4_481_107785 [pii]
AID - 10.4103/0377-4929.107785 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2012 Oct-Dec;55(4):481-4. doi: 
      10.4103/0377-4929.107785.