PMID- 23456144
OWN - NLM
STAT- MEDLINE
DCOM- 20130606
LR  - 20191210
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Linking)
VI  - 18
IP  - 6
DP  - 2013 Jun
TI  - In vivo detection of basal cell carcinoma: comparison of a reflectance confocal 
      microscope and a multiphoton tomograph.
PG  - 61229
LID - 10.1117/1.JBO.18.6.061229 [doi]
AB  - The standard diagnostic procedure for basal cell carcinoma (BCC) is invasive 
      tissue biopsy with time-consuming histological examination. To reduce the number 
      of biopsies, noninvasive optical methods have been developed providing 
      high-resolution skin examination. We present direct comparison of a reflectance 
      confocal microscope (RLSM) and a multiphoton tomograph (MPT) for BCC diagnosis. 
      Both systems are applied to nine patients prior to surgery, and the results are 
      analyzed, including histological results. Both systems prove suitable for 
      detecting typical characteristics of BCC in various stages. The RLSM allows large 
      horizontal overview images to be obtained, enabling the investigator to find the 
      regions of interest quickly, e.g., BCC nests. Elongated cells and palisading 
      structures are easily recognized using both methods. Due to the higher 
      resolution, changes in nucleus diameter or cytoplasm could be visualized with the 
      MPT. Therefore, the nucleus diameter, nucleus/cytoplasm ratio, and cell density 
      are estimated for normal and BCC cells using the MPT. The nucleus of elongated 
      BCC cells is significantly longer than other measured normal skin cells, whereas 
      the cell density and nucleus/cytoplasm ratio of BCC cannot be significantly 
      distinguished from granular cells.
FAU - Ulrich, Martina
AU  - Ulrich M
AD  - Department of Dermatology, Allergology and Venerology, 
      Charite-Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany.
FAU - Klemp, Marisa
AU  - Klemp M
FAU - Darvin, Maxim E
AU  - Darvin ME
FAU - Konig, Karsten
AU  - Konig K
FAU - Lademann, Jurgen
AU  - Lademann J
FAU - Meinke, Martina C
AU  - Meinke MC
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Basal Cell/*diagnosis/pathology
MH  - Cell Count
MH  - Cell Nucleus/pathology
MH  - Cell Nucleus Shape
MH  - Cell Nucleus Size
MH  - Cytoplasm/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Microscopy, Confocal/*methods
MH  - Microscopy, Fluorescence, Multiphoton/*methods
MH  - Middle Aged
MH  - Optical Phenomena
MH  - Skin Neoplasms/*diagnosis/pathology
MH  - Tomography, Optical/*methods
EDAT- 2013/03/05 06:00
MHDA- 2013/06/07 06:00
CRDT- 2013/03/05 06:00
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/06/07 06:00 [medline]
AID - 1659721 [pii]
AID - 10.1117/1.JBO.18.6.061229 [doi]
PST - ppublish
SO  - J Biomed Opt. 2013 Jun;18(6):61229. doi: 10.1117/1.JBO.18.6.061229.