PMID- 23456659
OWN - NLM
STAT- MEDLINE
DCOM- 20130613
LR  - 20141120
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 31
IP  - 6
DP  - 2013 Jun
TI  - Organ culture stability of the intervertebral disc: rat versus rabbit.
PG  - 838-46
LID - 10.1002/jor.22285 [doi]
AB  - There is a need to develop mechanically active culture systems to better 
      understand the role of mechanical stresses in intervertebral disc (IVD) 
      degeneration. Motion segment cultures that preserve the native IVD structure and 
      adjacent vertebral bodies are preferred as model systems, but rapid ex vivo 
      tissue degeneration limits their usefulness. The stability of rat and rabbit IVDs 
      is of particular interest, as their small size makes them otherwise suitable for 
      motion segment culture. The goal of this study was to determine if there are 
      substantial differences in the susceptibility of rat and rabbit IVDs to 
      culture-induced degeneration. Lumbar IVD motion segments were harvested from 
      young adult male Sprague-Dawley rats and New Zealand White rabbits and cultured 
      under standard conditions for 14 days. Biochemical assays and safranin-O 
      histology showed that while glycosaminoglycan (GAG) loss was minimal in rabbit 
      IVDs, it was progressive and severe in rat IVDs. In the rat IVD, GAG loss was 
      concomitant with the loss of notochordal cells and the migration of endplate (EP) 
      cells into the nucleus pulposus (NP). None of these changes were evident in the 
      rabbit IVDs. Compared to rabbit IVDs, rat IVDs also showed increased matrix 
      metalloproteinase-3 (MMP-3) and sharply decreased collagen type I and II collagen 
      expression. Together these data indicated that the rabbit IVD was dramatically 
      more stable than the rat IVD, which showed culture-related degenerative changes. 
      Based on these findings we conclude that the rabbit motion segments are a 
      superior model for mechanobiologic studies.
CI  - Copyright (c) 2013 Orthopaedic Research Society.
FAU - Seol, Dongrim
AU  - Seol D
AD  - Departments of Orthopedics and Rehabilitation, University of Iowa, Iowa City, 
      Iowa 52242, USA.
FAU - Choe, Hyeonghun
AU  - Choe H
FAU - Ramakrishnan, Prem S
AU  - Ramakrishnan PS
FAU - Jang, Keewoong
AU  - Jang K
FAU - Kurriger, Gail L
AU  - Kurriger GL
FAU - Zheng, Hongjun
AU  - Zheng H
FAU - Lim, Tae-Hong
AU  - Lim TH
FAU - Martin, James A
AU  - Martin JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20130301
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Glycosaminoglycans)
SB  - IM
MH  - Animals
MH  - Gene Expression
MH  - Glycosaminoglycans/*metabolism
MH  - Intervertebral Disc/anatomy & histology/*metabolism
MH  - Male
MH  - Models, Animal
MH  - *Organ Culture Techniques
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2013/03/05 06:00
MHDA- 2013/06/14 06:00
CRDT- 2013/03/05 06:00
PHST- 2012/03/02 00:00 [received]
PHST- 2012/11/05 00:00 [accepted]
PHST- 2013/03/05 06:00 [entrez]
PHST- 2013/03/05 06:00 [pubmed]
PHST- 2013/06/14 06:00 [medline]
AID - 10.1002/jor.22285 [doi]
PST - ppublish
SO  - J Orthop Res. 2013 Jun;31(6):838-46. doi: 10.1002/jor.22285. Epub 2013 Mar 1.