PMID- 23458164
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20211203
IS  - 2152-4998 (Electronic)
IS  - 2152-4971 (Print)
IS  - 2152-4971 (Linking)
VI  - 15
IP  - 2
DP  - 2013 Apr
TI  - Identifying candidate oocyte reprogramming factors using cross-species global 
      transcriptional analysis.
PG  - 126-33
LID - 10.1089/cell.2012.0060 [doi]
AB  - There is mounting evidence to suggest that the epigenetic reprogramming capacity 
      of the oocyte is superior to that of the current factor-based reprogramming 
      approaches and that some factor-reprogrammed induced pluripotent stem cells 
      (iPSCs) retain a degree of epigenetic memory that can influence differentiation 
      capacity and may be linked to the observed expression of immunogenicity genes in 
      iPSC derivatives. One hypothesis for this differential reprogramming capacity is 
      the "chromatin loosening/enhanced reprogramming" concept, as previously described 
      by John Gurdon and Ian Wilmut, as well as others, which postulates that the 
      oocyte possesses factors that loosen the somatic cell chromatin structure, 
      providing the epigenetic and transcriptional regulatory factors more ready access 
      to repressed genes and thereby significantly increasing epigenetic reprogramming. 
      However, to empirically test this hypothesis a list of candidate oocyte 
      reprogramming factors (CORFs) must be ascertained that are significantly 
      expressed in metaphase II oocytes. Previous studies have focused on intraspecies 
      or cross-species transcriptional analysis of up to two different species of 
      oocytes. In this study, we have identified eight CORFs (ARID2, ASF1A, ASF1B, 
      DPPA3, ING3, MSL3, H1FOO, and KDM6B) based on unbiased global transcriptional 
      analysis of oocytes from three different species (human, rhesus monkey, and 
      mouse) that both demonstrate significant (p<0.05, FC>3) expression in oocytes of 
      all three species and have well-established roles in loosening/opening up 
      chromatin structure. We also identified an additional 15 CORFs that fit within 
      our proposed "chromatin opening/fate transformative" (COFT) model. These CORFs 
      may be able to augment Shinya Yamanaka's previously identified reprogramming 
      factors (OCT4, SOX2, KLF4, and cMYC) and potentially facilitate the removal of 
      epigenetic memory in iPSCs and/or reduce the expression of immunogenicity genes 
      in iPSC derivatives, and may have applications in future personalized pluripotent 
      stem cell based therapeutics.
FAU - Awe, Jason P
AU  - Awe JP
AD  - Department of Molecular and Medical Pharmacology, University of California, Los 
      Angeles, CA 90095, USA.
FAU - Byrne, James A
AU  - Byrne JA
LA  - eng
GR  - UL1 TR000124/TR/NCATS NIH HHS/United States
GR  - UL1TR000124/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130304
PL  - United States
TA  - Cell Reprogram
JT  - Cellular reprogramming
JID - 101528176
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Cell Transdifferentiation
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Induced Pluripotent Stem Cells/cytology/*metabolism
MH  - Kruppel-Like Factor 4
MH  - Macaca mulatta
MH  - Mice
MH  - Oocytes/cytology/*metabolism
MH  - Species Specificity
MH  - *Transcription, Genetic
PMC - PMC3616412
EDAT- 2013/03/06 06:00
MHDA- 2013/09/24 06:00
PMCR- 2014/04/01
CRDT- 2013/03/06 06:00
PHST- 2013/03/06 06:00 [entrez]
PHST- 2013/03/06 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1089/cell.2012.0060 [pii]
AID - 10.1089/cell.2012.0060 [doi]
PST - ppublish
SO  - Cell Reprogram. 2013 Apr;15(2):126-33. doi: 10.1089/cell.2012.0060. Epub 2013 Mar 
      4.