PMID- 23458967
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20191210
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 27
IP  - 4
DP  - 2013 Jun
TI  - Artificial neural network analysis of data from multiple in vitro assays for 
      prediction of skin sensitization potency of chemicals.
PG  - 1233-46
LID - S0887-2333(13)00046-5 [pii]
LID - 10.1016/j.tiv.2013.02.013 [doi]
AB  - In order to develop in vitro risk assessment systems for skin sensitization, it 
      is important to predict a threshold from the murine local lymph node assay 
      (LLNA). We first confirmed that the combination of the human Cell Line Activation 
      Test (h-CLAT) and the SH test improved the accuracy and sensitivity of prediction 
      of LLNA data compared with each individual test. Next, we assessed the mutual 
      correlations among maximum amount of change of cell-surface thiols (MAC value) in 
      the SH test, CV75 value (concentration giving 75% cell viability) in a 
      cytotoxicity assay, EC150 and EC200 values (thresholds concentrations of CD86 and 
      CD54 expression, respectively) in h-CLAT and published LLNA thresholds of 64 
      chemicals. Based on the results, we selected MAC value and the minimum of CV75, 
      EC150 (CD86) and EC200 (CD54) as descriptors for the input layer of an artificial 
      neural network (ANN) system. The ANN-predicted values were well correlated with 
      reported LLNA thresholds. We also found a correlation between the SH test and the 
      peptide-binding assay used to evaluate hapten-protein complex formation. Thus, 
      this model, which we designate as the "iSENS ver. 1", may be useful for risk 
      assessment of skin sensitization potential of chemicals from in vitro test data.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Hirota, Morihiko
AU  - Hirota M
AD  - Shiseido Research Center, Shiseido Co Ltd, 2-12-1 Fukuura, Kanazawa-ku, 
      Yokohama-shi, Kanagawa 236-8643, Japan. morihiko.hirota@to.shiseido.co.jp
FAU - Kouzuki, Hirokazu
AU  - Kouzuki H
FAU - Ashikaga, Takao
AU  - Ashikaga T
FAU - Sono, Sakiko
AU  - Sono S
FAU - Tsujita, Kyoko
AU  - Tsujita K
FAU - Sasa, Hitoshi
AU  - Sasa H
FAU - Aiba, Setsuya
AU  - Aiba S
LA  - eng
PT  - Journal Article
DEP - 20130301
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Allergens)
RN  - 0 (Haptens)
RN  - 0 (Sulfhydryl Compounds)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Biological Assay
MH  - Cell Line
MH  - Haptens/*toxicity
MH  - Humans
MH  - Local Lymph Node Assay
MH  - Mice
MH  - *Neural Networks, Computer
MH  - Protein Binding
MH  - Sulfhydryl Compounds/metabolism
EDAT- 2013/03/06 06:00
MHDA- 2013/10/23 06:00
CRDT- 2013/03/06 06:00
PHST- 2012/10/04 00:00 [received]
PHST- 2013/02/18 00:00 [revised]
PHST- 2013/02/19 00:00 [accepted]
PHST- 2013/03/06 06:00 [entrez]
PHST- 2013/03/06 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - S0887-2333(13)00046-5 [pii]
AID - 10.1016/j.tiv.2013.02.013 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2013 Jun;27(4):1233-46. doi: 10.1016/j.tiv.2013.02.013. Epub 
      2013 Mar 1.