PMID- 23459692
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1616-301X (Print)
IS  - 1616-3028 (Electronic)
IS  - 1616-301X (Linking)
VI  - 21
IP  - 23
DP  - 2011 Dec 6
TI  - Preparation and Characterization of Molecularly Imprinted Polymeric Nanoparticles 
      for Atrial Natriuretic Peptide (ANP).
PG  - 4423-4429
AB  - Natriuretic peptide receptor A (NPRA), the receptor for the cardiac hormone 
      atrial natriuretic peptide (ANP), is expressed abundantly on cancer cells and 
      disruption of ANP-NPRA signaling inhibits tumor burden and metastasis. Since 
      antagonists of NPRA signaling have not provided reproducible results, we reasoned 
      that a synthetic neutralizing antibody to ANP, which has high selectivity and 
      affinity for ANP, could be used to regulate ANP levels and attenuate NPRA 
      signaling. In this study, we prepared molecularly imprinted polymer nanoparticles 
      (MIPNPs) for ANP using a short peptide of ANP as the template and determined 
      their binding affinity and selectivity. The MIPNPs were prepared by precipitation 
      polymerization using NH(2)-SLRRSS-CONH(2), which is a short peptide from ANP as 
      template, methacrylic acid (MAA) and N-isopropylacrylamide (NIPAm) as functional 
      monomers, bis-acrylamide (BIS) as crosslinker. The average diameter of MIPNPs and 
      non-imprinted nanoparticles (NIPNPs) in water is 215.8 +/-4.6 nm and 197.7+/-3.1 nm 
      respectively. The binding isotherm analysis showed that MIPNPs have a much higher 
      binding affinity for template peptide and ANP than NIPNPs. Scatchard analysis 
      gave an equilibrium dissociation constant, K(d) of 7.3 muM with a binding capacity 
      106.7 mumol/g for template peptide and K(d) of 7.9 muM with a binding capacity of 
      36.0 mumol/g for ANP. Measurements of binding kinetics revealed that MIPNPs reach 
      protein adsorption equilibrium in 30 min. MIPNPs found to have high specificity 
      for ANP with little affinity for BSA or scrambled ANP peptide. MIPNPs also 
      recognized and adsorbed ANP in cell culture media spiked with ANP and human 
      plasma. Taken together, these results indicate that MIPNPs have high affinity and 
      selectivity for ANP and can be used as a synthetic antibody for modulating 
      ANP-NPRA signaling in cancers.
FAU - Wang, Chunyan
AU  - Wang C
AD  - Department of Molecular Medicine, University of South Florida, 12901 Bruce B. 
      Downs Blvd. ; Nanomedicine Research Center, University of South Florida, 12901 
      Bruce B. Downs Blvd.
FAU - Howell, Mark
AU  - Howell M
FAU - Raulji, Payal
AU  - Raulji P
FAU - Davis, Yvonne
AU  - Davis Y
FAU - Mohapatra, Subhra
AU  - Mohapatra S
LA  - eng
GR  - R01 CA152005/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20110928
PL  - Germany
TA  - Adv Funct Mater
JT  - Advanced functional materials
JID - 101190390
PMC - PMC3583218
MID - NIHMS430880
OTO - NOTNLM
OT  - ANP-NPRA signaling
OT  - Atrial natriuretic peptide (ANP)
OT  - cancer
OT  - molecularly imprinted polymer
OT  - natriuretic peptide receptor A (NPRA)
EDAT- 2011/12/06 00:00
MHDA- 2011/12/06 00:01
PMCR- 2013/02/27
CRDT- 2013/03/06 06:00
PHST- 2013/03/06 06:00 [entrez]
PHST- 2011/12/06 00:00 [pubmed]
PHST- 2011/12/06 00:01 [medline]
PHST- 2013/02/27 00:00 [pmc-release]
AID - 10.1002/adfm.201100946 [doi]
PST - ppublish
SO  - Adv Funct Mater. 2011 Dec 6;21(23):4423-4429. doi: 10.1002/adfm.201100946. Epub 
      2011 Sep 28.