PMID- 23460839
OWN - NLM
STAT- MEDLINE
DCOM- 20130903
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 2
DP  - 2013
TI  - Elevated transcription of the gene QSOX1 encoding quiescin Q6 sulfhydryl oxidase 
      1 in breast cancer.
PG  - e57327
LID - 10.1371/journal.pone.0057327 [doi]
LID - e57327
AB  - The q arm of chromosome 1 is frequently amplified at the gene level in breast 
      cancer. Since the significance of this is unclear we investigated whether 1q 
      genes are overexpressed in this disease. The cDNA levels of 1q-located genes were 
      analysed in a search for overexpressed genes. 26 genes mapping to the 1q arm show 
      highly significant (P</=0.01) overexpression of transcripts in breast cancer 
      compared to normal breast tissue. Amongst those showing the highest levels of 
      overexpression in both expressed sequence tag (EST) and serial analysis of gene 
      expression (SAGE) databases was enzyme quiescin Q6 sulfhydryl oxidase 1 (QSOX1). 
      We investigated QSOX1 cDNA derived from T47D breast carcinoma cells by RT-PCR and 
      3'-RACE PCR and identified a novel extended form of QSOX1 transcript, containing 
      a long 3'UTR, nearly double the size of the previously reported QSOX1 cDNA, and 
      confirmed its 3' end nucleotide sequence using RACE-PCR. We also used 
      quantitative real-time PCR to analyse a panel of cDNAs derived from 50 
      clinically-graded normal and malignant breast tissue samples for the expression 
      of QSOX1 mRNAs. QSOX1 transcription was elevated in an increasing proportion in 
      the grade 2 and grade 3 tumours (graded according to the Nottingham prognostic 
      index), with 10 of the 15 grade 3 tumours (67%) examined exceeding the normal 
      range. There was a significant correlation between relative transcript level and 
      clinical grade (P</=0.01) for all qPCR primer sets tested. QSOX1 mRNA levels, based 
      on SAGE expression data, did not correlate with either Estrogen Receptor (ER) or 
      Epidermal Growth Factor Receptor 2 (ErbB-2 or HER2/neu) expression. Our data 
      indicate that QSOX1 is a potential new prognostic marker which may prove of use 
      in the staging of breast tumours and the stratification of breast cancer 
      patients.
FAU - Soloviev, Mikhail
AU  - Soloviev M
AD  - School of Biological Sciences, Centre for Biomedical Sciences, Royal Holloway 
      University of London, London, United Kingdom. mikhail.soloviev@rhul.ac.uk
FAU - Esteves, Michelle P
AU  - Esteves MP
FAU - Amiri, Fakhria
AU  - Amiri F
FAU - Crompton, Mark R
AU  - Crompton MR
FAU - Rider, Christopher C
AU  - Rider CC
LA  - eng
PT  - Journal Article
DEP - 20130227
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 1.8.- (Oxidoreductases Acting on Sulfur Group Donors)
RN  - EC 1.8.3.2 (QSOX1 protein, human)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Base Sequence
MH  - Breast Neoplasms/*enzymology/*genetics
MH  - Carcinoma, Ductal, Breast/enzymology/genetics
MH  - Cell Line, Tumor
MH  - DNA, Complementary/genetics
MH  - Expressed Sequence Tags
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Neoplasm/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - Oxidoreductases Acting on Sulfur Group Donors/*genetics/metabolism
MH  - Protein Biosynthesis/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - *Transcription, Genetic
MH  - Up-Regulation/genetics
PMC - PMC3583868
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/03/06 06:00
MHDA- 2013/09/04 06:00
PMCR- 2013/02/27
CRDT- 2013/03/06 06:00
PHST- 2012/09/16 00:00 [received]
PHST- 2013/01/21 00:00 [accepted]
PHST- 2013/03/06 06:00 [entrez]
PHST- 2013/03/06 06:00 [pubmed]
PHST- 2013/09/04 06:00 [medline]
PHST- 2013/02/27 00:00 [pmc-release]
AID - PONE-D-12-28133 [pii]
AID - 10.1371/journal.pone.0057327 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(2):e57327. doi: 10.1371/journal.pone.0057327. Epub 2013 Feb 27.