PMID- 23462619
OWN - NLM
STAT- MEDLINE
DCOM- 20130802
LR  - 20220317
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 72
DP  - 2013 Feb 19
TI  - The Citrobacter rodentium mouse model: studying pathogen and host contributions 
      to infectious colitis.
PG  - e50222
LID - 10.3791/50222 [doi]
LID - 50222
AB  - This protocol outlines the steps required to produce a robust model of infectious 
      disease and colitis, as well as the methods used to characterize Citrobacter 
      rodentium infection in mice. C. rodentium is a gram negative, murine specific 
      bacterial pathogen that is closely related to the clinically important human 
      pathogens enteropathogenic E. coli and enterohemorrhagic E. coli. Upon infection 
      with C. rodentium, immunocompetent mice suffer from modest and transient weight 
      loss and diarrhea. Histologically, intestinal crypt elongation, immune cell 
      infiltration, and goblet cell depletion are observed. Clearance of infection is 
      achieved after 3 to 4 weeks. Measurement of intestinal epithelial barrier 
      integrity, bacterial load, and histological damage at different time points after 
      infection, allow the characterization of mouse strains susceptible to infection. 
      The virulence mechanisms by which bacterial pathogens colonize the intestinal 
      tract of their hosts, as well as specific host responses that defend against such 
      infections are poorly understood. Therefore the C. rodentium model of enteric 
      bacterial infection serves as a valuable tool to aid in our understanding of 
      these processes. Enteric bacteria have also been linked to Inflammatory Bowel 
      Diseases (IBDs). It has been hypothesized that the maladaptive chronic 
      inflammatory responses seen in IBD patients develop in genetically susceptible 
      individuals following abnormal exposure of the intestinal mucosal immune system 
      to enteric bacteria. Therefore, the study of models of infectious colitis offers 
      significant potential for defining potentially pathogenic host responses to 
      enteric bacteria. C. rodentium induced colitis is one such rare model that allows 
      for the analysis of host responses to enteric bacteria, furthering our 
      understanding of potential mechanisms of IBD pathogenesis; essential in the 
      development of novel preventative and therapeutic treatments.
FAU - Bhinder, Ganive
AU  - Bhinder G
AD  - Division of Gastroenterology, BC Children's Hospital, Canada.
FAU - Sham, Ho Pan
AU  - Sham HP
FAU - Chan, Justin M
AU  - Chan JM
FAU - Morampudi, Vijay
AU  - Morampudi V
FAU - Jacobson, Kevan
AU  - Jacobson K
FAU - Vallance, Bruce A
AU  - Vallance BA
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20130219
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Citrobacter rodentium/*pathogenicity
MH  - Colitis/*microbiology
MH  - *Disease Models, Animal
MH  - Enterobacteriaceae Infections/*microbiology
MH  - Host-Pathogen Interactions
MH  - Mice
MH  - Virulence
PMC - PMC3605715
EDAT- 2013/03/07 06:00
MHDA- 2013/08/03 06:00
PMCR- 2015/02/19
CRDT- 2013/03/07 06:00
PHST- 2013/03/07 06:00 [entrez]
PHST- 2013/03/07 06:00 [pubmed]
PHST- 2013/08/03 06:00 [medline]
PHST- 2015/02/19 00:00 [pmc-release]
AID - 50222 [pii]
AID - 10.3791/50222 [doi]
PST - epublish
SO  - J Vis Exp. 2013 Feb 19;(72):e50222. doi: 10.3791/50222.