PMID- 23468097
OWN - NLM
STAT- MEDLINE
DCOM- 20140411
LR  - 20211021
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 30
IP  - 4
DP  - 2013 Apr
TI  - High-magnification sperm selection does not decrease the aneuploidy rate in 
      patients who are heterozygous for reciprocal translocations.
PG  - 525-30
LID - 10.1007/s10815-013-9959-3 [doi]
AB  - PROBLEM: This study sought to evaluate the value of motile sperm organelle 
      morphology examination (MSOME) for selecting euploid spermatozoa in six patients 
      who were heterozygous for a reciprocal translocation. METHOD OF STUDY: We used 
      sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the 
      chromosomes involved in the translocations and a putative interchromosomal effect 
      (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole 
      sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a 
      magnification typically used for intracytoplasmic sperm injection (ICSI), 
      referred to as "ICSI-like", and (iii) with MSOME. RESULTS: The balanced 
      translocation rates did not differ significantly (p=0.81) when comparing whole 
      sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME 
      (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly 
      (p=0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) 
      and MSOME-selected spermatozoa (1.0 %). CONCLUSION: For patients who are 
      heterozygous for reciprocal translocations, MSOME does not improve the selection 
      of euploid spermatozoa.
FAU - Chelli, Mohamed Hassen
AU  - Chelli MH
AD  - Department of Reproductive Biology, Cytogenetics, Gynaecology and Obstetrics, 
      CHIPS, Centre Hospitalier Poissy Saint-Germain, 10 rue Champ-Gaillard, 78303, 
      Poissy, France.
FAU - Ferfouri, Fatma
AU  - Ferfouri F
FAU - Boitrelle, Florence
AU  - Boitrelle F
FAU - Albert, Martine
AU  - Albert M
FAU - Molina-Gomes, Denise
AU  - Molina-Gomes D
FAU - Selva, Jacqueline
AU  - Selva J
FAU - Vialard, Francois
AU  - Vialard F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130307
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/genetics
MH  - Male
MH  - Middle Aged
MH  - Semen Analysis
MH  - Sperm Injections, Intracytoplasmic
MH  - Spermatozoa/*cytology/ultrastructure
MH  - Translocation, Genetic
PMC - PMC3644130
EDAT- 2013/03/08 06:00
MHDA- 2014/04/12 06:00
PMCR- 2014/04/01
CRDT- 2013/03/08 06:00
PHST- 2012/12/24 00:00 [received]
PHST- 2013/02/11 00:00 [accepted]
PHST- 2013/03/08 06:00 [entrez]
PHST- 2013/03/08 06:00 [pubmed]
PHST- 2014/04/12 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 9959 [pii]
AID - 10.1007/s10815-013-9959-3 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2013 Apr;30(4):525-30. doi: 10.1007/s10815-013-9959-3. 
      Epub 2013 Mar 7.