PMID- 23469541
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR  - 20131121
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 29
IP  - 6
DP  - 2012 Dec
TI  - [Biomechanic and biological activity assessment of concavity-convex amniotic 
      membrane].
PG  - 1114-8
AB  - This paper conducted research on biomechanical characteristics and biological 
      activity of concavity-convex amniotic membrane (CCAM) and discussed its 
      superiority as ocular surface repair material. Folding and compression with 
      vacuum of fresh amniotic membrane were used to prepare CCAM. After cutting the 
      striga of CCAM, sixteen CCAM tissue section were chosen at random to test their 
      tensile strength using electronic universal testing machine. The bilayer amniotic 
      membrane (BAM), the double-deck amniotic membrane (DAM) and the monolayer 
      amniotic membrane (MAM) were as controls. The test parameters included yield 
      strength, tensile strength, elongation at break, elastic modulus and so on. The 
      cytokines of fresh amniotic membrane (FAM), MAM and CCAM were analyzed by 
      radioimmunoassay method. The CCAM was obviously thicker than MAM and DAM. After 
      15 min in PBS, the CCAM tissue can recover the normal shape. The tensile strength 
      and the elongation at break of CCAM were higher than those of the MAM and the DAM 
      (P < 0.05). The elastic modulus of the CCAM was smaller than that of the MAM and 
      the DAM (P < 0.05). The content of 10 cytokines [epidermal growth factor (EGF), 
      fibroblast growth factor (FGF), b-fibroblast growth factor b-FGF, hepatocyte 
      growth factor (HGF), transforming growth factor-beta (TGF-beta), insulin-like 
      growth factor (IGF), platelet-derived growth factor (PDGF), vascular endothelial 
      growth factor (VEGF), nerve growth factor (NGF), brain-derived nellrotrophic 
      factor (BDNF), ciliary neurotrophic factor (CNTF)] of CCAM decreased 
      significantly compared with the FAM and increased significantly compared with MAM 
      and DAM in 6 cytokines (EGF, FGF, HGF, TGF-betap, PDGF, NGF; P < 0.05). The CCAM 
      composites is thinner and has higher cytokine content than MAM, and better 
      biomechanical properties than the MAM and the DAM, showing the superiority as 
      ocular surface repair material.
FAU - Shao, Yi
AU  - Shao Y
AD  - Department of Ophthalmology, First Affiliated Hospital of Nanchang University, 
      Nanchang 330006, China. Freebee992296@yahoo.com.cn
FAU - Yu, Yao
AU  - Yu Y
FAU - Tan, Gang
AU  - Tan G
FAU - Peng, Juan
AU  - Peng J
FAU - Zhou, Qiong
AU  - Zhou Q
FAU - Pei, Chonggang
AU  - Pei C
FAU - Dong, Wenjia
AU  - Dong W
FAU - Gao, Guiping
AU  - Gao G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Cytokines)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Amnion/*chemistry/physiology/*transplantation
MH  - Biomechanical Phenomena
MH  - Cytokines/*analysis
MH  - Epidermal Growth Factor/analysis
MH  - Fibroblast Growth Factors/analysis
MH  - Hepatocyte Growth Factor/analysis
MH  - Humans
MH  - Tissue Engineering/methods
MH  - *Tissue Scaffolds
EDAT- 2013/03/09 06:00
MHDA- 2013/11/01 06:00
CRDT- 2013/03/09 06:00
PHST- 2013/03/09 06:00 [entrez]
PHST- 2013/03/09 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2012 Dec;29(6):1114-8.