PMID- 23471014
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20171116
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 75
IP  - 4
DP  - 2013 Apr
TI  - Differences in phosphodiesterase 3A and 3B expression after ischemic insult.
PG  - 340-8
LID - S0168-0102(13)00040-0 [pii]
LID - 10.1016/j.neures.2013.02.006 [doi]
AB  - Phosphodiesterase (PDE) exists in the cardiovascular system, adipose tissue and 
      platelets, and its inhibition increases the cellular levels of cAMP, which could 
      activate cAMP-responsive element binding protein (pCREB). The present study was 
      designed to map the expression of PDE3A/B in the forebrain and define the time 
      course of PDE3 expression in the ischemic boundary zone after ischemia. The 
      number of PDE3A-positive cells (neurons and endothelial cells) remained 
      unchanged, while PDE3B-positive cells gradually increased after 
      ischemia/reperfusion. In the corpus callosum, PDE3B was expressed in 
      oligodendrocytes, oligodendrocyte progenitor cells, and astrocytes. 
      PDE3B-expressing astrocytes showed gradual increase after ischemia/reperfusion. 
      In the cortex, the majority of PDE3B-expressing cells before ischemia were 
      neurons, though few were astrocytes. Ischemic insult resulted in gradual increase 
      in PDE3B-expressing astrocytes and neurons, with larger increase in astrocytes. 
      Expression of brain derived neurotrophic factor (BDNF) and B-cell 
      leukemia/lymphoma 2 protein (Bcl-2) was detected in pCREB-positive cells, not in 
      PDE3B-positive cells. Our results demonstrated that ischemic insult increased 
      PDE3B expression, but not PDE3A, and changed the number and type of cells in a 
      time-dependent manner. The variation of PDE3B-expression in the brain might play 
      a crucial pathophysiological role, and regulation of PDE3B production might 
      protect against ischemic brain damage.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All 
      rights reserved.
FAU - Mitome-Mishima, Yumiko
AU  - Mitome-Mishima Y
AD  - Department of Neurosurgery, Juntendo University School of Medicine, Tokyo, Japan.
FAU - Miyamoto, Nobukazu
AU  - Miyamoto N
FAU - Tanaka, Ryota
AU  - Tanaka R
FAU - Oishi, Hidenori
AU  - Oishi H
FAU - Arai, Hajime
AU  - Arai H
FAU - Hattori, Nobutaka
AU  - Hattori N
FAU - Urabe, Takao
AU  - Urabe T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130305
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Aif1 protein, mouse)
RN  - 0 (CD11b Antigen)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Microfilament Proteins)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 3)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/*enzymology/pathology
MH  - CD11b Antigen/metabolism
MH  - CREB-Binding Protein/metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Cell Count
MH  - Cerebral Cortex/enzymology/pathology
MH  - Cerebrovascular Circulation/physiology
MH  - Corpus Callosum/enzymology/pathology
MH  - Cyclic Nucleotide Phosphodiesterases, Type 3/*metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - In Situ Nick-End Labeling
MH  - Infarction, Middle Cerebral Artery/*enzymology/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microfilament Proteins/metabolism
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Phosphorylation
MH  - Reperfusion
EDAT- 2013/03/09 06:00
MHDA- 2013/10/01 06:00
CRDT- 2013/03/09 06:00
PHST- 2012/10/22 00:00 [received]
PHST- 2013/01/31 00:00 [revised]
PHST- 2013/02/04 00:00 [accepted]
PHST- 2013/03/09 06:00 [entrez]
PHST- 2013/03/09 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
AID - S0168-0102(13)00040-0 [pii]
AID - 10.1016/j.neures.2013.02.006 [doi]
PST - ppublish
SO  - Neurosci Res. 2013 Apr;75(4):340-8. doi: 10.1016/j.neures.2013.02.006. Epub 2013 
      Mar 5.