PMID- 23475428
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 38
IP  - 5
DP  - 2013 May
TI  - Neural stem-like cells derived from human amnion tissue are effective in treating 
      traumatic brain injury in rat.
PG  - 1022-33
LID - 10.1007/s11064-013-1012-5 [doi]
AB  - Although human amnion derived mesenchymal stem cells (AMSC) are a promising 
      source of stem cells, their therapeutic potential for traumatic brain injury 
      (TBI) has not been widely investigated. In this study, we evaluated the 
      therapeutic potential of AMSC using a rat TBI model. AMSC were isolated from 
      human amniotic membrane and characterized by flow cytometry. After induction, 
      AMSC differentiated in vitro into neural stem-like cells (AM-NSC) that expressed 
      higher levels of the neural stem cell markers, nestin, sox2 and musashi, in 
      comparison to undifferentiated AMSC. Interestingly, the neurotrophic factors, 
      brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin 
      3 (NT-3), glial cell derived neurotrophic factor (GDNF) and ciliary neurotrophic 
      factor (CNTF) were markedly upregulated after neural stem cell induction. 
      Following transplantation in a rat TBI model, significant improvements in 
      neurological function, brain tissue morphology, and higher levels of BDNF, NGF, 
      NT-3, GDNF and CNTF, were observed in the AM-NSC group compared with the AMSC and 
      Matrigel groups. However, few grafted cells survived with minimal differentiation 
      into neural-like cells. Together, our results suggest that transplantation of 
      AM-NSC promotes functional rehabilitation of rats with TBI, with enhanced 
      expression of neurotrophic factors a likely mechanistic pathway.
FAU - Yan, Zhong-Jie
AU  - Yan ZJ
AD  - Key Laboratory on Brain Function Repair and Regeneration of Guangdong, 
      Neurosurgery Institute, Zhujiang Hospital, Southern Medical University, Guangzhou 
      510282, China.
FAU - Zhang, Peng
AU  - Zhang P
FAU - Hu, Yu-Qin
AU  - Hu YQ
FAU - Zhang, Hong-Tian
AU  - Zhang HT
FAU - Hong, Sun-Quan
AU  - Hong SQ
FAU - Zhou, Hong-Long
AU  - Zhou HL
FAU - Zhang, Mao-Ying
AU  - Zhang MY
FAU - Xu, Ru-Xiang
AU  - Xu RX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130310
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (DNA Primers)
SB  - IM
MH  - Amnion/*cytology
MH  - Animals
MH  - Base Sequence
MH  - Brain Injuries/*therapy
MH  - Cell Differentiation
MH  - DNA Primers
MH  - Female
MH  - Humans
MH  - Neural Stem Cells/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2013/03/12 06:00
MHDA- 2013/10/23 06:00
CRDT- 2013/03/12 06:00
PHST- 2012/11/27 00:00 [received]
PHST- 2013/03/01 00:00 [accepted]
PHST- 2013/02/24 00:00 [revised]
PHST- 2013/03/12 06:00 [entrez]
PHST- 2013/03/12 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - 10.1007/s11064-013-1012-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2013 May;38(5):1022-33. doi: 10.1007/s11064-013-1012-5. Epub 2013 
      Mar 10.