PMID- 23475571
OWN - NLM
STAT- MEDLINE
DCOM- 20140106
LR  - 20130626
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 110
IP  - 8
DP  - 2013 Aug
TI  - Segregated flux balance analysis constrained by population structure/function 
      data: the case of PHA production by mixed microbial cultures.
PG  - 2267-76
LID - 10.1002/bit.24894 [doi]
AB  - In this study we developed a segregated flux balance analysis (FBA) method to 
      calculate metabolic flux distributions of the individual populations present in a 
      mixed microbial culture (MMC). Population specific flux data constraints were 
      derived from the raw data typically obtained by the fluorescence in situ 
      hybridization (FISH) and microautoradiography (MAR)-FISH techniques. This method 
      was applied to study the metabolic heterogeneity of a MMC that produces 
      polyhydroxyalkanoates (PHA) from fermented sugar cane molasses. Three populations 
      were identified by FISH, namely Paracoccus sp., Thauera sp., and Azoarcus sp. The 
      segregated FBA method predicts a flux distribution for each of the identified 
      populations. The method is shown to predict with high accuracy the average PHA 
      storage flux and the respective monomeric composition for 16 independent 
      experiments. Moreover, flux predictions by segregated FBA were slightly better 
      than those obtained by nonsegregated FBA, and also highly concordant with 
      metabolic flux analysis (MFA) estimated fluxes. The segregated FBA method can be 
      of high value to assess metabolic heterogeneity in MMC systems and to derive more 
      efficient eco-engineering strategies. For the case of PHA-producing MMC 
      considered in this work, it becomes apparent that the PHA average monomeric 
      composition might be controlled not only by the volatile fatty acids (VFA) 
      feeding profile but also by the population composition present in the MMC.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Pardelha, F
AU  - Pardelha F
AD  - REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 
      Caparica, Portugal.
FAU - Albuquerque, M G E
AU  - Albuquerque MG
FAU - Carvalho, G
AU  - Carvalho G
FAU - Reis, M A M
AU  - Reis MA
FAU - Dias, J M L
AU  - Dias JM
FAU - Oliveira, R
AU  - Oliveira R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130503
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (DNA, Bacterial)
RN  - 0 (Polyhydroxyalkanoates)
SB  - IM
MH  - Azoarcus/classification/genetics/*metabolism
MH  - Bioreactors/*microbiology
MH  - *Biota
MH  - DNA, Bacterial/genetics
MH  - Fermentation
MH  - In Situ Hybridization, Fluorescence
MH  - *Microbial Consortia
MH  - Molasses
MH  - Paracoccus/classification/genetics/*metabolism
MH  - Polyhydroxyalkanoates/*biosynthesis
MH  - Saccharum/metabolism
MH  - Thauera/classification/genetics/*metabolism
EDAT- 2013/03/12 06:00
MHDA- 2014/01/07 06:00
CRDT- 2013/03/12 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/02/25 00:00 [revised]
PHST- 2013/02/26 00:00 [accepted]
PHST- 2013/03/12 06:00 [entrez]
PHST- 2013/03/12 06:00 [pubmed]
PHST- 2014/01/07 06:00 [medline]
AID - 10.1002/bit.24894 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2013 Aug;110(8):2267-76. doi: 10.1002/bit.24894. Epub 2013 May 
      3.