PMID- 23475608
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20161125
IS  - 1098-2744 (Electronic)
IS  - 0899-1987 (Linking)
VI  - 53
IP  - 8
DP  - 2014 Aug
TI  - Thromboxane A2 receptor-mediated release of matrix metalloproteinase-1 (MMP-1) 
      induces expression of monocyte chemoattractant protein-1 (MCP-1) by activation of 
      protease-activated receptor 2 (PAR2) in A549 human lung adenocarcinoma cells.
PG  - 659-66
LID - 10.1002/mc.22020 [doi]
AB  - Matrix metalloproteinases (MMPs) and monocyte chemoattractant protein-1 (MCP-1, 
      CCL2) are known to be upregulated in many tumors. Their roles in tumor invasion 
      and metastasis are being uncovered. How they are related to each other and 
      involved in tumor progression remains to be determined. Earlier it was reported 
      that I-BOP-initiated activation of thromboxane A2 receptor (TP) induced the 
      release of MMP-1, MMP-3, and MMP-9 from lung cancer A549 cells overexpressing TPalpha 
      (A549-TPalpha). Herein it was found that MMP-1, but not MMP-3 or MMP-9, induced the 
      expression of MCP-1 in A549 cells. Conditioned medium (CM) from I-BOP activated, 
      MMP-1 siRNA pretreated A549-TPalpha cells induced greatly attenuated expression of 
      MCP-1 in A549 cells indicating that MMP-1 in the CM contributed significantly to 
      the expression of MCP-1. MMP-1 was shown to activate protease-activated receptor 
      2 (PAR2) instead of commonly assumed PAR1 to increase the expression of MCP-1 in 
      A549 cells. This conclusion was reached from the following findings: (1) 
      expression of MCP-1 induced by trypsin, a PAR2 agonist, and also PAR2 agonist 
      peptide, was inhibited by a PAR2 antagonist; (2) expression of MCP-1 induced by 
      MMP-1 and by CM from I-BOP activated A549-TPalpha cells was blocked by a PAR2 
      antagonist but not by other PAR antagonists; (3) expression of MCP-1 induced by 
      MMP-1 and by CM from I-BOP activated A549-TPalpha cells was attenuated significantly 
      by pretreatment of cells with PAR2-siRNA. These results suggest that PAR2 is a 
      novel MMP-1 target mediating MMP-1-induced signals in A549 lung cancer cells.
CI  - (c) 2013 Wiley Periodicals, Inc.
FAU - Li, Xiuling
AU  - Li X
AD  - Department of Pharmaceutical Sciences, College of Pharmacy, University of 
      Kentucky, Lexington, Kentucky.
FAU - Tai, Hsin-Hsiung
AU  - Tai HH
LA  - eng
PT  - Journal Article
DEP - 20130308
PL  - United States
TA  - Mol Carcinog
JT  - Molecular carcinogenesis
JID - 8811105
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptor, PAR-2)
RN  - 0 (Receptors, Thromboxane A2, Prostaglandin H2)
RN  - 124924-85-2 
      (7-(3-(3-hydroxy-4-(4'-iodophenoxy)-1-butenyl)-7-oxabicyclo(2.2.1)heptan-2-yl)-5-heptenoic 
      acid)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Adenocarcinoma/drug therapy/genetics/*metabolism
MH  - Blotting, Western
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Culture Media, Conditioned/pharmacology
MH  - Fatty Acids, Unsaturated/pharmacology
MH  - Humans
MH  - Lung Neoplasms/drug therapy/genetics/*metabolism
MH  - Matrix Metalloproteinase 1/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, PAR-2/antagonists & inhibitors/genetics/*metabolism
MH  - Receptors, Thromboxane A2, Prostaglandin H2/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - matrix metalloproteinases
OT  - monocyte chemoattractant protein-1
OT  - protease-activated receptor 2
OT  - thromboxane A2 receptor
EDAT- 2013/03/12 06:00
MHDA- 2014/09/12 06:00
CRDT- 2013/03/12 06:00
PHST- 2012/09/06 00:00 [received]
PHST- 2013/01/24 00:00 [revised]
PHST- 2013/02/04 00:00 [accepted]
PHST- 2013/03/12 06:00 [entrez]
PHST- 2013/03/12 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
AID - 10.1002/mc.22020 [doi]
PST - ppublish
SO  - Mol Carcinog. 2014 Aug;53(8):659-66. doi: 10.1002/mc.22020. Epub 2013 Mar 8.