PMID- 23478084
OWN - NLM
STAT- MEDLINE
DCOM- 20131001
LR  - 20141120
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 183
IP  - 2
DP  - 2013 Aug
TI  - CD1d blockade suppresses the capacity of immature dendritic cells to prime 
      allogeneic T cell response.
PG  - 894-9
LID - S0022-4804(13)00084-X [pii]
LID - 10.1016/j.jss.2013.01.066 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the principal antigen-presenting cells 
      involved in primary immune response and immunoregulation. The function of DCs is 
      believed to depend on their degree of maturation. Mature DCs activate immune 
      responses, whereas immature DCs (imDCs) tend to induce immune tolerance. CD1 is 
      involved in regulating the development of imDCs, which have important roles in 
      initiating or suppressing the immune response after transplantation. MATERIALS 
      AND METHODS: We used male BALB/c mice and C57BL/6 mice (aged 8-10 wk, 18-22 g). 
      We isolated and purified T lymphocytes from mouse spleen. Immature DCs modified 
      by viral delivery of interleukin-10 (IL-10) were stimulated with granulocyte 
      macrophage colony-stimulating factor and lipopolysaccharide (LPS) and treated 
      with anti-CD1d in vitro. We used mixed lymphocyte cultures to evaluate the 
      heterogeneity of T lymphocyte response. We also examined the proliferation of T 
      lymphocytes and the expression of cytokines. RESULTS: CD1d blockade did not 
      impair granulocyte macrophage colony-stimulating factor and LPS-stimulated DC 
      maturation. We observed a dramatic increase in allogeneic T lymphocyte 
      proliferation (stimulation index) at all tested responder-stimulator ratios in 
      response to imDCs cultured in the presence of LPS (P < 0.05). CD1d has an 
      important role in imDC-primed T cell response (P < 0.05). CD1d blockade reduced 
      the capacity of imDCs to prime allogeneic T cells. T cells pre-sensitized by 
      LPS-stimulated imDCs showed remarkably elevated proliferation in response to T 
      cells from either BALB/c or C57BL/6 mice (P < 0.01). We observed a significant 
      decrease in the proliferation of T cells pre-sensitized by stimulated imDCs after 
      CD1d blockade. Lipopolysaccharide stimulation caused elevated the production of 
      IL-12 and tumor necrosis factor-alpha (TNF-alpha) (P < 0.01) and decreased the secretion 
      of IL-10 (P < 0.05). The addition of CD1d neutralization antibody did not 
      significantly change the concentrations of IL-12, TNF-alpha, or IL-10 produced by 
      imDCs cultured in the presence of LPS (P > 0.05). CONCLUSIONS: Blockade of CD1d 
      impaired the ability of imDCs to stimulate allogeneic T cell response. By reduced 
      T cell proliferation, the secretion of IL-12 and TNF-alpha decreased and production 
      of a T-helper type 2 cytokine IL-10 increased, which indicates the potential of 
      CD1d blockade as a method to induce immune tolerance to allograft antigens in 
      transplantation.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Ma, Zhao-Hui
AU  - Ma ZH
AD  - Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical 
      University, Xi'an, People's Republic of China.
FAU - Lu, Hang
AU  - Lu H
FAU - Lu, Qiang
AU  - Lu Q
FAU - Yao, Zhi-Fa
AU  - Yao ZF
FAU - Han, Yong
AU  - Han Y
LA  - eng
PT  - Journal Article
DEP - 20130222
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD1d)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/*pharmacology
MH  - Antigens, CD1d/*drug effects/immunology/*physiology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/drug effects/*physiology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Immune Tolerance/drug effects/physiology
MH  - In Vitro Techniques
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - T-Lymphocytes/*cytology/drug effects
MH  - Transplantation, Homologous
OTO - NOTNLM
OT  - CD1d
OT  - Dendritic cells
OT  - Immune tolerance
OT  - Transplantation
EDAT- 2013/03/13 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/03/13 06:00
PHST- 2012/10/21 00:00 [received]
PHST- 2012/12/27 00:00 [revised]
PHST- 2013/01/31 00:00 [accepted]
PHST- 2013/03/13 06:00 [entrez]
PHST- 2013/03/13 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - S0022-4804(13)00084-X [pii]
AID - 10.1016/j.jss.2013.01.066 [doi]
PST - ppublish
SO  - J Surg Res. 2013 Aug;183(2):894-9. doi: 10.1016/j.jss.2013.01.066. Epub 2013 Feb 
      22.