PMID- 23478262
OWN - NLM
STAT- MEDLINE
DCOM- 20130617
LR  - 20211021
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 33
IP  - 10
DP  - 2013 May
TI  - The AP-1 complex regulates intracellular localization of insulin receptor 
      substrate 1, which is required for insulin-like growth factor I-dependent cell 
      proliferation.
PG  - 1991-2003
LID - 10.1128/MCB.01394-12 [doi]
AB  - The activation of the insulin/insulin-like growth factor I (IGF-I) receptor and 
      the subsequent tyrosine phosphorylation of insulin receptor substrates (IRSs) are 
      key initial events in a variety of insulin/IGF bioactivities, including 
      mitogenesis. It has been reported that IRS-1 associates with intracellular 
      membrane compartments, and this localization is believed to be important for 
      insulin/IGF signal transduction. However, the molecular mechanisms underlying 
      IRS-1 localization remain unclear. Here we show that in L6 myoblasts, IRS-1 
      associates with mu1A of the ubiquitously expressed AP-1 complex, which packages 
      cargo proteins into clathrin-coated vesicles derived from intracellular 
      membranes. While wild-type IRS-1 was predominantly localized to vesicular 
      structures, IRS-1 mutants lacking three YXXPhi motifs responsible for binding to 
      mu1A were mislocalized to the mannose-6-phosphate receptor-positive structures, 
      suggesting that AP-1-dependent transport to peripheral vesicles is inhibited in 
      these mutants. Furthermore, deletion of AP-1 binding sites in IRS-1 impaired 
      IGF-I-induced cell proliferation, accompanied by reduced tyrosine phosphorylation 
      of IRS-1 and its association with phosphoinositide (PI) 3-kinase. These data 
      demonstrate the importance of AP-1-dependent localization of IRS-1 in mediating 
      IGF-I-stimulated signaling and maximum mitogenic response.
FAU - Yoneyama, Yosuke
AU  - Yoneyama Y
AD  - Department of Animal Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
FAU - Matsuo, Masao
AU  - Matsuo M
FAU - Take, Kazumi
AU  - Take K
FAU - Kabuta, Tomohiro
AU  - Kabuta T
FAU - Chida, Kazuhiro
AU  - Chida K
FAU - Hakuno, Fumihiko
AU  - Hakuno F
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130311
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (AP1M1 protein, human)
RN  - 0 (Adaptor Protein Complex 1)
RN  - 0 (Adaptor Protein Complex mu Subunits)
RN  - 0 (IRS1 protein, human)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Protein Subunits)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Adaptor Protein Complex 1/*metabolism
MH  - Adaptor Protein Complex mu Subunits/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Membrane/metabolism
MH  - *Cell Proliferation
MH  - DNA Replication
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - Insulin Receptor Substrate Proteins/chemistry/*metabolism
MH  - Insulin-Like Growth Factor I/*physiology
MH  - Molecular Sequence Data
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Interaction Domains and Motifs
MH  - Protein Processing, Post-Translational
MH  - Protein Subunits
MH  - Protein Transport
MH  - Rats
MH  - Time-Lapse Imaging
PMC - PMC3647963
EDAT- 2013/03/13 06:00
MHDA- 2013/06/19 06:00
PMCR- 2013/11/01
CRDT- 2013/03/13 06:00
PHST- 2013/03/13 06:00 [entrez]
PHST- 2013/03/13 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - MCB.01394-12 [pii]
AID - 01394-12 [pii]
AID - 10.1128/MCB.01394-12 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2013 May;33(10):1991-2003. doi: 10.1128/MCB.01394-12. Epub 2013 
      Mar 11.