PMID- 23482588 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220311 IS - 2162-2000 (Print) IS - 2162-2019 (Electronic) IS - 2162-2019 (Linking) VI - 2 IP - 2 DP - 2012 May 29 TI - Statins Enhance Expression of Growth Factors and Activate the PI3K/Akt-mediated Signaling Pathway after Experimental Intracerebral Hemorrhage. PG - 74-80 AB - Previous studies have demonstrates that statins improve neurological outcome and promote neurovascular recovery after ICH. This study is designed to examine whether simvastatin and atorvastatin affect levels of growth factors and activate the Akt signaling pathway during the recovery phase after intracerebral hemorrhage (ICH) in rats. Sixty (60) male Wistar rats were subjected to ICH by stereotactic injection of 100 muL of autologous blood into the striatum and were treated with or without simvastatin or atorvastatin. Neurological functional outcome was evaluated by behavioral tests (mNSS and corner turn test) at different time points after ICH. Brain extracts were utilized for Enzyme-linked immunosorbent assay (ELISA) analyses to measure vascular endothelial growth factor (VEGF); brain-derived neurotrophin factor (BDNF) expression, and nerve growth factor (NGF). Western blot was used to measure the changes in the Akt-mediated signaling pathway. Both the simvastatin- and atorvastatin-treated animals had significant neurological improvement at 2 weeks post-ICH. Simvastatin and atorvastatin treatment increased the expression of BDNF, VEGF and NGF in both low- and high-dose groups at 7 days after ICH (p < 0.05). Phosphorylation of Akt, glycogen synthase kinase-3beta (GSK-3beta), and cAMP response element-binding proteins (CREB) were also increased at 7 days after statin treatment. These results suggest that the therapeutic effects of statins after experimental ICH may be mediated by the transient induction of BDNF, VEGF and NGF expression and the activation of the Akt-mediated signaling pathway. FAU - Yang, Dongmei AU - Yang D AD - Department of Neurosurgery, Henry Ford Health System, Detroit MI. FAU - Han, Yuxia AU - Han Y FAU - Zhang, Jianfeng AU - Zhang J FAU - Chopp, Michael AU - Chopp M FAU - Seyfried, Donald M AU - Seyfried DM LA - eng GR - R01 NS058581/NS/NINDS NIH HHS/United States PT - Journal Article PL - United States TA - World J Neurosci JT - World journal of neuroscience JID - 101585688 PMC - PMC3589759 MID - NIHMS380455 OTO - NOTNLM OT - Statin OT - experimental OT - growth factor OT - intracerebral hemorrhage EDAT- 2013/03/14 06:00 MHDA- 2013/03/14 06:01 PMCR- 2013/05/29 CRDT- 2013/03/14 06:00 PHST- 2013/03/14 06:00 [entrez] PHST- 2013/03/14 06:00 [pubmed] PHST- 2013/03/14 06:01 [medline] PHST- 2013/05/29 00:00 [pmc-release] AID - 10.4236/wjns.2012.22011 [doi] PST - ppublish SO - World J Neurosci. 2012 May 29;2(2):74-80. doi: 10.4236/wjns.2012.22011.