PMID- 23483219
OWN - NLM
STAT- MEDLINE
DCOM- 20131029
LR  - 20131121
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 31
IP  - 5
DP  - 2013 May
TI  - Changes in glutamate homeostasis cause retinal degeneration in Royal College of 
      Surgeons rats.
PG  - 1075-80
LID - 10.3892/ijmm.2013.1297 [doi]
AB  - The aim of the present study was to investigate glutamate homeostasis in retinal 
      degeneration-induced changes and the potential mechanisms of glutamate-mediated 
      excitotoxicity in a rat model. The expression of vesicular glutamate 
      transporter-1 (VGLUT-1) and protein kinase Calpha (PKCalpha) in wild-type and Royal 
      College of Surgeons (RCS) rat retinas, at postnatal Day 15 (P15), P30, P60 and 
      P90, were detected using quantitative real-time polymerase chain reaction and 
      immunohistochemistry. The levels of glutamine synthetase (GS) and 
      L-glutamate/L-aspartate transporter (GLAST) were evaluated by western blotting. 
      Compared with wild-type rats, outer nuclear layer thickness was significantly 
      thinner and VGLUT-1 expression was upregulated in a time-dependent pattern in RCS 
      rats. The ratio of VGLUT-1 to PKCalpha in RCS rats peaked at P60 (p<0.01) and 
      subsequently decreased by P90 (p<0.01), while it remained constant in wild-type 
      rats. The expression of GS increased gradually from P30 to P90 in RCS rats 
      (p<0.01), while it remained constant in wild-type rats at various time-points. No 
      significant difference in GLAST expression was found between RCS and wild-type 
      rats at all stages of retinal degeneration. Our results confirm the occurrence of 
      glutamate-mediated excitotoxicity to RCS rat retinas and provide an experimental 
      foundation for safeguarding the remnant visual function in retinal degenerative 
      disorders.
FAU - Liu, Kang
AU  - Liu K
AD  - Southwest Eye Hospital, Southwest Hospital, The Third Military Medical 
      University, Chongqing 400038, P.R. China.
FAU - Wang, Yi
AU  - Wang Y
FAU - Yin, Zhengqin
AU  - Yin Z
FAU - Weng, Chuanhuang
AU  - Weng C
FAU - Zeng, Yuxiao
AU  - Zeng Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130312
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc17a7 protein, rat)
RN  - 0 (Slc1a3 protein, rat)
RN  - 0 (Vesicular Glutamate Transport Protein 1)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Blotting, Western
MH  - Densitometry
MH  - Excitatory Amino Acid Transporter 1/metabolism
MH  - Fluorescent Antibody Technique
MH  - Glutamate-Ammonia Ligase/metabolism
MH  - Glutamic Acid/*metabolism
MH  - *Homeostasis
MH  - Protein Kinase C-alpha/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Retinal Degeneration/enzymology/*metabolism/*pathology
MH  - Vesicular Glutamate Transport Protein 1/genetics/metabolism
EDAT- 2013/03/14 06:00
MHDA- 2013/10/30 06:00
CRDT- 2013/03/14 06:00
PHST- 2012/09/24 00:00 [received]
PHST- 2012/11/09 00:00 [accepted]
PHST- 2013/03/14 06:00 [entrez]
PHST- 2013/03/14 06:00 [pubmed]
PHST- 2013/10/30 06:00 [medline]
AID - 10.3892/ijmm.2013.1297 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 May;31(5):1075-80. doi: 10.3892/ijmm.2013.1297. Epub 2013 Mar 
      12.