PMID- 23484703
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20181023
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 21
IP  - 1
DP  - 2013 Feb
TI  - [Detection of chromosome abnormalities in myelodysplastic syndromes with interval 
      fluorescence in situ hybridization].
PG  - 116-20
LID - 10.7534/j.issn.1009-2137.2013.01.024 [doi]
AB  - This study was aimed to investigate the value of interval fluorescence in situ 
      hybridization (FISH) in detection of abnormal karyotypes of patients with 
      myelodysplastic syndromes (MDS). Conventional cytogenetics (CC) and interval FISH 
      methods were carried out to analyze the bone marrow cells in 80 cases of MDS and 
      20 normal people. The results showed that using FISH, 53.8% cases of MDS (43/80) 
      were found with abnormal karyotypes which was higher than 21.3% detected by CC 
      method. There was significant difference between the 2 methods in detecting 
      abnormal karyotypes in MDS (P < 0.05). Among all World Health Organization (WHO) 
      subtypes, more chromosome abnormal were detected by FISH than by CC, especially 
      for refractory anemia (RA) and refractory cytopenia with multilineage dysplasia 
      (RCMD) groups. The detecting rate in patients with intermediate risk of 
      International Prognostic Scoring System (IPSS) also had a statistical difference 
      between FISH and CC methods. It is concluded that the FISH is more sensitive than 
      CC in detection of abnormal karyotypes in MDS and is informative for the cases 
      with karyotype failure or normal karyotype tested by CC. It is mainly embodied in 
      the intermediate risk cases of IPSS. In addition, patients with RA and RCMD may 
      benefit more from FISH for diagnosis compared with other WHO subtypes.
FAU - Ru, Xiao
AU  - Ru X
AD  - Department of Hematology, Provincial Hospital Affiliated to Shandong University, 
      Jinan, Shandong Province, China.
FAU - Li, Qing
AU  - Li Q
FAU - Fang, Xiao-Sheng
AU  - Fang XS
FAU - Li, Ying
AU  - Li Y
FAU - Wang, Xin
AU  - Wang X
FAU - Zhang, Ling-Yan
AU  - Zhang LY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/diagnosis/*genetics
MH  - Young Adult
EDAT- 2013/03/15 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/03/15 06:00
PHST- 2013/03/15 06:00 [entrez]
PHST- 2013/03/15 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - 1009-2137(2013)01-0116-05 [pii]
AID - 10.7534/j.issn.1009-2137.2013.01.024 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Feb;21(1):116-20. doi: 
      10.7534/j.issn.1009-2137.2013.01.024.