PMID- 23484957
OWN - NLM
STAT- MEDLINE
DCOM- 20140228
LR  - 20130603
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 38
IP  - 7
DP  - 2013 Jul
TI  - Expression profiling in glaucomatous human lamina cribrosa cells based on 
      graph-clustering approach.
PG  - 767-73
LID - 10.3109/02713683.2013.770039 [doi]
AB  - PURPOSE: In primary open angle glaucoma (POAG) patients, elevated intraocular 
      pressure usually leads to extracellular matrix remodeling and astrocytes 
      activation. Thus, lamina cribrosa (LC) cells may play an important role in POAG 
      progression. The objective of this study was to comprehensively explore gene 
      expression profiles in LC cells of POAG patients. MATERIALS AND METHODS: Using 
      the GSE13534 microarray datasets downloaded from Gene Expression Omnibus 
      database, the differentially expressed genes (DEGs) between LC cells from POAG 
      patients and controls were firstly screened based on the classical t-test and 
      false discovery rate <0.05 as a significant threshold. Subsequently, these DEGs 
      were grouped into gene sets using a graph-clustering approach. The underlying 
      molecular mechanisms were investigated by the Gene Ontology and Kyoto 
      Encyclopedia of Genes and Genomes pathway enrichment analysis. RESULTS: A total 
      of 57 DEGs were identified and 478 co-expression relationships were constructed 
      among these DEGs. Among them, cytochrome p450 family 1 subfamily B (CYP1B1), 
      brain-derived neurotrophic factor (BDNF) and myelin basic protein (MBP) showed 
      high-degree relationships and they could interact with several genes. CYP1B1 is 
      an important genetic gene involved in POAG and BDNF is an effective growth 
      neurotrophic factor to weak POAG damage. MBP, versican (VCAN), integrin, alpha 4 
      (ITGA4) and N-cadherin (CDH2) may be involved in extracellular matrix remodeling 
      in LC cells. FZD2 and FZD7 were enriched in basal cell carcinoma pathway. 
      CONCLUSIONS: The results demonstrate that the genes above may be associated with 
      the pathogenesis of POAG.
FAU - Luo, Dawei
AU  - Luo D
AD  - Division of Ophthalmology, The First Affiliated People's Hospital, Shanghai 
      Jiaotong University, Shanghai, China.
FAU - Liu, Kun
AU  - Liu K
FAU - Zhu, Bijun
AU  - Zhu B
FAU - Xu, Xun
AU  - Xu X
LA  - eng
PT  - Journal Article
DEP - 20130313
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cadherins)
RN  - 0 (Extracellular Matrix Proteins)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cadherins/genetics/metabolism
MH  - Cells, Cultured
MH  - Cluster Analysis
MH  - Extracellular Matrix/metabolism
MH  - Extracellular Matrix Proteins/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - Glaucoma, Open-Angle/*genetics/pathology
MH  - Humans
MH  - Intraocular Pressure
MH  - Oligonucleotide Array Sequence Analysis
MH  - Optic Disk/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2013/03/15 06:00
MHDA- 2014/03/01 06:00
CRDT- 2013/03/15 06:00
PHST- 2013/03/15 06:00 [entrez]
PHST- 2013/03/15 06:00 [pubmed]
PHST- 2014/03/01 06:00 [medline]
AID - 10.3109/02713683.2013.770039 [doi]
PST - ppublish
SO  - Curr Eye Res. 2013 Jul;38(7):767-73. doi: 10.3109/02713683.2013.770039. Epub 2013 
      Mar 13.