PMID- 23485057
OWN - NLM
STAT- MEDLINE
DCOM- 20130826
LR  - 20220330
IS  - 1743-1328 (Electronic)
IS  - 0161-6412 (Linking)
VI  - 35
IP  - 3
DP  - 2013 Apr
TI  - Transplantation of differentiated bone marrow stromal cells promotes motor 
      functional recovery in rats with stroke.
PG  - 320-8
LID - 10.1179/1743132812Y.0000000151 [doi]
AB  - OBJECTIVE: To investigate the effects of transplantation into rats with stroke of 
      bone marrow-derived mesenchymal stem cells (MSCs) induced by brain-derived 
      neurotrophic factor (BDNF). MATERIALS AND METHODS: MSCs were harvested from 
      6-week-old Sprague-Dawley male rats, and transplanted into adult Sprague-Dawley 
      male rats with transient middle cerebral artery occlusion. At 48 hours after 
      stroke, the adult rats, weighing 250-280 g, were injected via the tail vein with 
      1x10(7) MSCs or MSCs induced by BDNF (BDNF-MSCs) suspended in 1 ml 
      phosphate-buffered saline. All animals underwent the neurological function test 
      for 14 days. Infarct volume and blood-brain barrier permeability assay were 
      assessed on day 14 post-middle cerebral artery occlusion. Western blotting and 
      immunohistochemical staining were used to detect the protein expression of 
      neuron-specific enolase (NSE). RESULTS: Both BDNF-MSCs and MSCs produced 
      improvement in neurological deficits compared with vehicle controls on day 14 
      (P<0.05). In particular, the group transplanted with BDNF-MSCs exhibited 
      significant recovery of motor function compared with the group transplanted with 
      MSCs alone (P<0.05). Both BDNF-MSCs and MSCs, but particularly the former, 
      reduced infarct volume, improved blood-brain barrier dysfunction, reduced serum 
      NSE levels, activated the NSE activity, and inhibited neuronal apoptosis in the 
      ischemic boundary zone. CONCLUSIONS: BDNF-MSCs might contribute to the motor 
      function improvement partly by reducing neuronal damage via upregulating the NSE 
      expression level and inhibiting neuronal apoptosis.
FAU - Huang, Wen
AU  - Huang W
AD  - Department of Neurology, First Affiliated Hospital, Guangxi Medical University, 
      Nanning 530021, China. hwen1229@yahoo.com.cn
FAU - Mo, Xuean
AU  - Mo X
FAU - Qin, Chao
AU  - Qin C
FAU - Zheng, Jinou
AU  - Zheng J
FAU - Liang, Zhijian
AU  - Liang Z
FAU - Zhang, Cheng
AU  - Zhang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Cell Differentiation/physiology
MH  - Disease Models, Animal
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Motor Activity
MH  - Phosphopyruvate Hydratase/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Recovery of Function
MH  - Stroke/pathology/*surgery
EDAT- 2013/03/15 06:00
MHDA- 2013/08/27 06:00
CRDT- 2013/03/15 06:00
PHST- 2013/03/15 06:00 [entrez]
PHST- 2013/03/15 06:00 [pubmed]
PHST- 2013/08/27 06:00 [medline]
AID - 10.1179/1743132812Y.0000000151 [doi]
PST - ppublish
SO  - Neurol Res. 2013 Apr;35(3):320-8. doi: 10.1179/1743132812Y.0000000151.