PMID- 23485802
OWN - NLM
STAT- MEDLINE
DCOM- 20131113
LR  - 20131121
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 240
DP  - 2013 Jun 14
TI  - Satellite glia cells in dorsal root ganglia express functional NMDA receptors.
PG  - 135-46
LID - S0306-4522(13)00165-6 [pii]
LID - 10.1016/j.neuroscience.2013.02.031 [doi]
AB  - Satellite glia cells (SGCs), within the dorsal root ganglia (DRG), surround the 
      somata of most sensory neurons. SGCs have been shown to interact with sensory 
      neurons and appear to be involved in the processing of afferent information. We 
      found that in rat DRG various N-methyl-D-aspartate receptor (NMDAr) subunits were 
      expressed in SGCs in intact ganglia and in vitro. In culture, when SGCs were 
      exposed to brief pulses of NMDA they evoked transient increases in cytoplasmic 
      calcium that were inhibited by specific NMDA blockers (MK-801, AP5) while they 
      were Mg(2)(+) insensitive indicating that SGCs express functional NMDAr. The 
      percentage of NMDA responsive SGCs was similar in mixed- (SGCs plus neurons) and 
      SGC-enriched cultures. The pattern of the magnitude changes of the NMDA-evoked 
      response was similar in SGCs and DRG neurons when they were in close proximity, 
      suggesting that the NMDA response of SGCs and DRG neurons is modulated by their 
      interactions. Treating the cultures with nerve growth factor, and/or 
      prostaglandin E(2) did not alter the percentage of SGCs that responded to NMDA. 
      Since glutamate appears to be released within the DRG, the detection of 
      functional NMDAr in SGCs suggests that their NMDAr activity could contribute to 
      the interactions between neurons and SGCs. In summary we demonstrated for the 
      first time that SGCs express functional NMDAr.
CI  - Copyright (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Castillo, C
AU  - Castillo C
AD  - Unidad de Neurociencias, Instituto de Estudios Avanzados IDEA, Apartado 17606, 
      Caracas 1015-A, Venezuela. cjcastillo.w@gmail.com
FAU - Norcini, M
AU  - Norcini M
FAU - Martin Hernandez, L A
AU  - Martin Hernandez LA
FAU - Correa, G
AU  - Correa G
FAU - Blanck, T J J
AU  - Blanck TJ
FAU - Recio-Pinto, E
AU  - Recio-Pinto E
LA  - eng
PT  - Journal Article
DEP - 20130226
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Dinoprostone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Ganglia, Spinal/*cytology
MH  - Glutamate-Ammonia Ligase/metabolism
MH  - Male
MH  - N-Methylaspartate/pharmacology
MH  - Nerve Growth Factor/pharmacology
MH  - Nerve Tissue Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Satellite Cells, Perineuronal/drug effects/*metabolism
EDAT- 2013/03/15 06:00
MHDA- 2013/11/14 06:00
CRDT- 2013/03/15 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2013/01/29 00:00 [revised]
PHST- 2013/02/13 00:00 [accepted]
PHST- 2013/03/15 06:00 [entrez]
PHST- 2013/03/15 06:00 [pubmed]
PHST- 2013/11/14 06:00 [medline]
AID - S0306-4522(13)00165-6 [pii]
AID - 10.1016/j.neuroscience.2013.02.031 [doi]
PST - ppublish
SO  - Neuroscience. 2013 Jun 14;240:135-46. doi: 10.1016/j.neuroscience.2013.02.031. 
      Epub 2013 Feb 26.