PMID- 23486965
OWN - NLM
STAT- MEDLINE
DCOM- 20130506
LR  - 20211021
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 33
IP  - 11
DP  - 2013 Mar 13
TI  - Oligodendroglial expression of TrkB independently regulates myelination and 
      progenitor cell proliferation.
PG  - 4947-57
LID - 10.1523/JNEUROSCI.3990-12.2013 [doi]
AB  - The neurotrophin brain-derived neurotrophic factor (BDNF) has been implicated in 
      regulating CNS myelination. BDNF mutant mice exhibit a hypomyelinating phenotype, 
      and BDNF exerts distinct effects upon oligodendroglial proliferation, 
      differentiation, and myelination in vitro. To investigate the precise influence 
      that BDNF exerts in regulating CNS myelination in vivo, we have generated 
      conditional knock-out mice in which TrkB has been deleted specifically in 
      oligodendrocytes. Deletion of TrkB disrupted normal oligodendrocyte myelination, 
      resulting in a significant reduction in myelin protein expression and myelination 
      of CNS white matter tracts during development. Importantly, conditional knock-out 
      mice exhibited normal numbers of mature oligodendrocytes and normal numbers of 
      myelinated axons; however, myelin thickness was significantly reduced during 
      development. These data indicate that while TrkB expression in oligodendrocytes 
      plays no role in the initial contact with axons, it exerts an important influence 
      in subsequent stages to promote myelin ensheathment. The conditional knock-out 
      mice also exhibited an increased density of oligodendrocyte progenitor cells 
      (OPCs) in CNS white matter tracts. Concordant with these results, in vitro 
      analyses using OPCs subjected to TrkB knockdown also revealed increased OPC 
      proliferation. Our data suggested this effect was dependent upon TrkC and p75 
      expression. Thus, our data demonstrate that TrkB expression in oligodendroglia 
      exerts a direct effect on oligodendrocytes to promote myelination and an indirect 
      effect upon the OPC population, modifying their proliferative potential.
FAU - Wong, Agnes W
AU  - Wong AW
AD  - Centre for Neuroscience Research, The University of Melbourne, Victoria 3010, 
      Australia.
FAU - Xiao, Junhua
AU  - Xiao J
FAU - Kemper, Dennis
AU  - Kemper D
FAU - Kilpatrick, Trevor J
AU  - Kilpatrick TJ
FAU - Murray, Simon S
AU  - Murray SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Autophagy-Related Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Rb1cc1 protein, mouse)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor alpha)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
SB  - IM
MH  - Age Factors
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Autophagy-Related Proteins
MH  - Cell Differentiation/genetics
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Central Nervous System/growth & development/metabolism
MH  - Coculture Techniques
MH  - Female
MH  - Ganglia, Spinal/cytology/metabolism
MH  - Gene Expression Regulation/genetics
MH  - Green Fluorescent Proteins/genetics
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Microscopy, Electron, Transmission
MH  - Myelin Basic Protein/genetics/metabolism
MH  - Myelin Sheath/*physiology/ultrastructure
MH  - Oligodendroglia/*metabolism/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Nerve Growth Factor/metabolism
MH  - Receptor, Platelet-Derived Growth Factor alpha/metabolism
MH  - Receptor, trkB/genetics/*metabolism
MH  - Receptor, trkC/metabolism
MH  - Receptor-Interacting Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Stem Cells/*physiology/ultrastructure
MH  - Transfection
PMC - PMC6619007
EDAT- 2013/03/15 06:00
MHDA- 2013/05/07 06:00
PMCR- 2013/09/13
CRDT- 2013/03/15 06:00
PHST- 2013/03/15 06:00 [entrez]
PHST- 2013/03/15 06:00 [pubmed]
PHST- 2013/05/07 06:00 [medline]
PHST- 2013/09/13 00:00 [pmc-release]
AID - 33/11/4947 [pii]
AID - 3827133 [pii]
AID - 10.1523/JNEUROSCI.3990-12.2013 [doi]
PST - ppublish
SO  - J Neurosci. 2013 Mar 13;33(11):4947-57. doi: 10.1523/JNEUROSCI.3990-12.2013.