PMID- 23488153
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20161125
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 30
IP  - 1
DP  - 2013 Feb
TI  - [Construction and identification of lentiviral vector of siRNA specific for 
      Beclin1 gene].
PG  - 131-5
AB  - The lentiviral vector was used for construction of a recombinant mediating RNA 
      interference (RNAi) against Beclin1 gene in this study. Recombinant vector 
      plasmid was transfected into non small cell lung cancer (NSCLC) A549 cells by 
      liposome. PCR results showed that three amplified positive fragments were 
      inserted into pRNAT-U6. 2/Lenti vectors. DNA sequencing results showed that the 
      three recombinant lentivirus plasmids, pRNAT-U6. 2/Lenti-si356, pRNAT-U6. 
      2/Lenti-si423 and pRNAT-U6. 2/ Lenti-si684 were constructed successfully. After 
      transfection with liposome, RT-PCR and Western blot analysis confirmed that the 
      expression of Beclin1 mRNA and protein was inhibited in the three recombinant 
      lentivirus plasmids transfected groups, and gene silencing efficacy was 35.56%, 
      89.22% and 66.78%, respectively. The results demonstrated that the lentiviral 
      vectors of RNAi targeting Beclin1 gene were successfully constructed, and NSCLC 
      A549 stable cell line with Beclin1 gene knockdown was established. This study 
      finally provided a new cell model to explore the biological behavior of the 
      Beclin1 gene in NSCLC A549 cells.
FAU - Wang, Wenyu
AU  - Wang W
AD  - Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan 430023, China.
FAU - Zhao, Guoqiang
AU  - Zhao G
FAU - Zhou, Yun
AU  - Zhou Y
FAU - Fan, Hongkun
AU  - Fan H
FAU - Wu, Gang
AU  - Wu G
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Autophagy/genetics
MH  - Base Sequence
MH  - Beclin-1
MH  - Carcinoma, Non-Small-Cell Lung/genetics/pathology
MH  - Cell Line, Tumor
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Lentivirus/*genetics
MH  - Lung Neoplasms/*genetics/pathology
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - RNA Interference
MH  - RNA, Small Interfering/*genetics
MH  - Transfection
EDAT- 2013/03/16 06:00
MHDA- 2014/05/13 06:00
CRDT- 2013/03/16 06:00
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Feb;30(1):131-5.