PMID- 23489681
OWN - NLM
STAT- MEDLINE
DCOM- 20131108
LR  - 20130426
IS  - 1532-3129 (Electronic)
IS  - 0021-9975 (Linking)
VI  - 148
IP  - 4
DP  - 2013 May
TI  - Pathogenesis of Korean type 1 (European genotype) porcine reproductive and 
      respiratory syndrome virus in experimentally infected pregnant gilts.
PG  - 396-404
LID - S0021-9975(12)00160-0 [pii]
LID - 10.1016/j.jcpa.2012.09.004 [doi]
AB  - The aims of this study were to determine (1) the pathogenesis of experimental 
      infection with a Korean type 1 porcine reproductive and respiratory syndrome 
      virus (PRRSV) by defining the viral distribution and the sites of viral 
      replication and (2) the relationship between viral replication and apoptosis in 
      stillborn fetuses and live born piglets from infected pregnant gilts. At 3 weeks 
      ante partum, four pregnant gilts were inoculated intranasally with Korean type 1 
      PRRSV. Stillborn fetuses from the infected gilts were of crown-to-rump length 
      25.8-27.1 cm consistent with fetal death between 106 and 110 days of gestation. 
      Type 1 PRRSV was isolated from the fetal tissues and these isolates were shown to 
      be identical to the challenge virus by sequence analysis. Type 1 PRRSV RNA was 
      detected in the lung, lymph node, heart, tonsil, thymus, liver, adrenal gland and 
      spleen of live born piglets and stillborn fetuses from the infected gilts. The 
      mean number of apoptotic cells per unit area of lung (P = 0.003), heart (P = 
      0.011), thymus (P = 0.003), liver (P = 0.011) and spleen (P = 0.002) was 
      significantly higher in stillborn fetuses than in live born piglets. Dual 
      labelling showed that the majority of cells either contained type 1 PRRSV or were 
      apoptotic, but not both. Apoptotic cells were more numerous than PRRSV(+) cells. 
      The results of the study demonstrated that type 1 PRRSV induces reproductive 
      failure in pregnant gilts. Apoptosis induced by type 1 PRRSV may be associated 
      with the incidence of stillborn fetuses in PRRSV-infected pregnant gilts.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Han, K
AU  - Han K
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Seo, H W
AU  - Seo HW
FAU - Oh, Y
AU  - Oh Y
FAU - Kang, I
AU  - Kang I
FAU - Park, C
AU  - Park C
FAU - Ha, B C
AU  - Ha BC
FAU - Kim, S-H
AU  - Kim SH
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130313
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Female
MH  - Fetal Death/pathology/*veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/isolation & 
      purification/*pathogenicity
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/pathology/*veterinary/virology
MH  - Swine
EDAT- 2013/03/16 06:00
MHDA- 2013/11/10 06:00
CRDT- 2013/03/16 06:00
PHST- 2012/07/28 00:00 [received]
PHST- 2012/08/29 00:00 [revised]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2013/11/10 06:00 [medline]
AID - S0021-9975(12)00160-0 [pii]
AID - 10.1016/j.jcpa.2012.09.004 [doi]
PST - ppublish
SO  - J Comp Pathol. 2013 May;148(4):396-404. doi: 10.1016/j.jcpa.2012.09.004. Epub 
      2013 Mar 13.