PMID- 23489844
OWN - NLM
STAT- MEDLINE
DCOM- 20140206
LR  - 20130819
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 197
IP  - 2
DP  - 2013 Aug
TI  - Effect of saliva stabilisers on detection of porcine reproductive and respiratory 
      syndrome virus in oral fluid by quantitative reverse transcriptase real-time PCR.
PG  - 224-8
LID - S1090-0233(13)00063-4 [pii]
LID - 10.1016/j.tvjl.2013.02.001 [doi]
AB  - This study evaluated the effect of extraction-amplification methods, storage 
      temperature and saliva stabilisers on detection of porcine reproductive and 
      respiratory syndrome virus (PRRSV) RNA by quantitative reverse transcriptase 
      real-time PCR (qRT-PCR) in porcine oral fluid. The diagnostic performance of 
      different extraction-amplification methods was examined using a dilution series 
      of oral fluid spiked with PRRSV. To determine RNA stability, porcine oral fluid, 
      with or without commercially available saliva stabilisers, was spiked with PRRSV, 
      stored at 4 degrees C or room temperature and tested for the presence of PRRSV RNA by 
      qRT-PCR. PRRSV RNA could be detected in oral fluid using all 
      extraction-amplification combinations, but the limit of detection varied amongst 
      different combinations. Storage temperature and saliva stabilisers had an effect 
      on the stability of PRRSV RNA, which could only be detected for 7 days when PRRSV 
      spiked oral fluid was kept at 4 degrees C or stabilised at room temperature with a 
      commercial mRNA stabiliser.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Decorte, Inge
AU  - Decorte I
AD  - Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, 
      CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium. Inge.Decorte@coda-cerva.be
FAU - Van der Stede, Yves
AU  - Van der Stede Y
FAU - Nauwynck, Hans
AU  - Nauwynck H
FAU - De Regge, Nick
AU  - De Regge N
FAU - Cay, Ann Brigitte
AU  - Cay AB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130313
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Excipients)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Excipients/*chemistry
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - RNA, Viral/isolation & purification
MH  - Real-Time Polymerase Chain Reaction/methods/*veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods/*veterinary
MH  - Saliva/chemistry/*virology
MH  - Specimen Handling
MH  - Swine
OTO - NOTNLM
OT  - Oral fluid
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Quantitative reverse transcriptase real-time PCR
EDAT- 2013/03/16 06:00
MHDA- 2014/02/07 06:00
CRDT- 2013/03/16 06:00
PHST- 2012/10/18 00:00 [received]
PHST- 2013/01/31 00:00 [revised]
PHST- 2013/02/04 00:00 [accepted]
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2014/02/07 06:00 [medline]
AID - S1090-0233(13)00063-4 [pii]
AID - 10.1016/j.tvjl.2013.02.001 [doi]
PST - ppublish
SO  - Vet J. 2013 Aug;197(2):224-8. doi: 10.1016/j.tvjl.2013.02.001. Epub 2013 Mar 13.