PMID- 23493369
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130318
LR  - 20211021
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 14
IP  - 4
DP  - 2011 Jul
TI  - Isolation and phenotyping of normal mouse liver dendritic cells by an improved 
      method.
PG  - 354-60
AB  - INTRODUCTION: Dendritic cells (DCs) are bone marrow-derived cells, which migrate 
      to lymphoid and non-lymphoid organs via blood. Liver DCs are believed to play an 
      important role in the regulation of hepatic allograft acceptance. However, 
      because of inherent difficulties in isolating adequate numbers of DCs from liver, 
      limited information is available on the phenotype and functions of liver DCs. To 
      address this issue, we isolated DCs from normal C57BL/6 mouse liver using a 
      modified procedure and described their immunophenotypic characteristics. 
      MATERIALS AND METHODS: Non-parenchymal cells (NPCs) were obtained by collagenase 
      digestion of perfused liver fragments and density gradient centrifugation (14.5% 
      nycodenz column). After overnight (18 hr) incubation of the NPCs, enrichment for 
      transiently adherent, low- density cells on 13% nycodenz gradients permitted the 
      recovery of low numbers of cells (approximately 1.2-1.5 x 10(5) per liver), many 
      of which displayed distinct DCs morphology (abundant cytoplasm with prominent 
      projections and irregularly shaped nuclei). RESULTS: Flowcytometric analysis 
      revealed that most of these cells were recognized by anti-CD11c (60-70%). The 
      results obtained from double staining with PE and FITC conjugated monoclonal 
      antibodies indicated that these cells were CD11c(+)/MHC-II(+) (53%), 
      CD11c(+)/CD86(+) (53.5%), CD11c(+)/ CD8alpha(+) (36%) and CD11c(+)/CD11b(+) (45%). 
      CONCLUSION: These findings indicate that the purity of DCs isolated by nycodenz 
      gradient is higher than other reported methods. Considering the similar ratio of 
      lymphoid (CD11c(+)/CD8alpha(+)) and myeloid (CD11c(+)/CD11b(+)) DCs in the liver, and 
      the known role of lymphoid DCs in tolerance induction, it seems that this 
      subpopulation of DCs is not the main reason of liver tolerogenecity. Therefore, 
      other factors such as the immaturity of liver DCs or the effect of liver 
      microenvironment on these cells, etc. may explain the acceptance of hepatic 
      allograft.
FAU - Mosayebi, Ghasem
AU  - Mosayebi G
AD  - Molecular and Medicine Research Center, School of Medicine, Arak University of 
      Medical Sciences, Arak, Iran.
FAU - Moazzeni, Sayyed Mohammad
AU  - Moazzeni SM
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC3586834
OTO - NOTNLM
OT  - Dendritic cells
OT  - Liver
OT  - Mice
OT  - Phenotype
EDAT- 2011/07/01 00:00
MHDA- 2011/07/01 00:01
PMCR- 2011/07/01
CRDT- 2013/03/16 06:00
PHST- 2010/05/22 00:00 [received]
PHST- 2011/02/02 00:00 [accepted]
PHST- 2013/03/16 06:00 [entrez]
PHST- 2011/07/01 00:00 [pubmed]
PHST- 2011/07/01 00:01 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - IJBMS-14-354 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2011 Jul;14(4):354-60.