PMID- 23493467
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130318
LR  - 20211021
IS  - 0972-124X (Print)
IS  - 0975-1580 (Electronic)
IS  - 0972-124X (Linking)
VI  - 16
IP  - 4
DP  - 2012 Oct
TI  - Estimation of matrix metalloproteinase-3 levels in gingival crevicular fluid in 
      periodontal disease, health and after scaling and root planing.
PG  - 549-52
LID - 10.4103/0972-124X.106907 [doi]
AB  - BACKGROUND: Initial research has shown a positive correlation between the 
      severity of periodontal disease and matrix metalloproteinase-3 (MMP-3) 
      concentrations in gingival crevicular fluid (GCF). However, there are no enough 
      reports to correlate the MMP-3 concentrations in GCF in periodontal health, 
      disease and after treatment. Hence, the present study is to estimate the levels 
      of MMP-3 in GCF in periodontal health, disease and to evaluate the effect of 
      periodontal therapy on MMP-3 concentrations in GCF. MATERIALS AND METHODS: 
      Periodontal examination and collection of GCF by extracrevicular method was 
      performed in 30 subjects selected randomly and categorized into three groups. 
      Group I (Healthy, n=10), group II (Chronic periodontitis, n=20) and group III 
      (After treatment group, n=20). Scaling and root planing (SRP) was performed and 
      GCF was collected after 8 weeks of treatment. MMP-3 levels were estimated in GCF 
      samples using enzyme linked immunosorbent assay (ELISA). RESULTS: MMP-3 was 
      detected in all samples. Highest mean MMP-3 concentrations in GCF were obtained 
      for group II (7.490 ng/ml), while the lowest concentrations were seen in group I 
      (0.344 ng/ml) and group III (2.129 ng/ml). This suggests that MMP-3 levels in GCF 
      increases proportionally with the progression of periodontal disease and 
      decreases after treatment. CONCLUSION: There is a substantial increase in the 
      concentrations of MMP-3 as periodontal disease progresses. Since MMP-3 levels in 
      GCF are positively correlated with gingival index, probing pocket depth and 
      clinical attachment level, MMP-3 may be considered as a "novel biomarker" in 
      periodontal disease progression. However, controlled, longitudinal studies are 
      needed to confirm this possibility.
FAU - Reddy, N Ravindra
AU  - Reddy NR
AD  - Department of Periodontics, CKS Teja Institute of Dental Sciences, Tirupati, 
      Andhra Pradesh, India.
FAU - Roopa, D
AU  - Roopa D
FAU - Babu, D S Madhu
AU  - Babu DS
FAU - Kumar, P Mohan
AU  - Kumar PM
FAU - Raju, C Mahendra
AU  - Raju CM
FAU - Kumar, N Sarath
AU  - Kumar NS
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Indian Soc Periodontol
JT  - Journal of Indian Society of Periodontology
JID - 101499342
PMC - PMC3590725
OTO - NOTNLM
OT  - Gingival crevicular fluid
OT  - matrix metalloproteinase-3
OT  - periodontal disease
OT  - scaling and root planing
COIS- Conflict of Interest: None declared.
EDAT- 2013/03/16 06:00
MHDA- 2013/03/16 06:01
PMCR- 2012/10/01
CRDT- 2013/03/16 06:00
PHST- 2011/03/05 00:00 [received]
PHST- 2012/09/16 00:00 [accepted]
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2013/03/16 06:01 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - JISP-16-549 [pii]
AID - 10.4103/0972-124X.106907 [doi]
PST - ppublish
SO  - J Indian Soc Periodontol. 2012 Oct;16(4):549-52. doi: 10.4103/0972-124X.106907.