PMID- 23494315
OWN - NLM
STAT- MEDLINE
DCOM- 20130826
LR  - 20130315
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 983
DP  - 2013
TI  - Transcript localization in Dictyostelium discoideum cells by RNA FISH.
PG  - 311-23
LID - 10.1007/978-1-62703-302-2_17 [doi]
AB  - Biogenesis of ribosomal RNA (rRNA) takes place preliminary in the nucleolus of 
      eukaryotic cells, the site of rDNA transcription. Several processing steps of 
      rRNA molecules have been implied to take place in the cytoplasm. To follow these 
      processing events we have adapted protocols for fluorescence in situ 
      hybridization (FISH) for use in Dictyostelium discoideum. We describe methods for 
      the generation of suitable fluorescently labeled probes and the fixation of 
      cells, by which we have localized different precursor and mature rRNA molecules 
      to the nucleolus or the cytoplasm, respectively.
FAU - Hofmann, Patrick
AU  - Hofmann P
AD  - Heisenberg Research Group Ribogenetics, Technical University of Darmstadt, 
      Darmstadt, Germany.
FAU - Kruse, Janis
AU  - Kruse J
FAU - Hammann, Christian
AU  - Hammann C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Protozoan)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Chromatography, Gel
MH  - Culture Techniques
MH  - DNA Probes/genetics/isolation & purification
MH  - DNA, Ribosomal/genetics
MH  - Dictyostelium/genetics/*metabolism
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Protozoan/genetics/*metabolism
MH  - RNA, Ribosomal/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 2013/03/16 06:00
MHDA- 2013/08/27 06:00
CRDT- 2013/03/16 06:00
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2013/08/27 06:00 [medline]
AID - 10.1007/978-1-62703-302-2_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;983:311-23. doi: 10.1007/978-1-62703-302-2_17.