PMID- 23495241
OWN - NLM
STAT- MEDLINE
DCOM- 20141110
LR  - 20160511
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 6
IP  - 3
DP  - 2014 Mar
TI  - Circulating microRNA as a biomarker of human growth hormone administration to 
      patients.
PG  - 234-8
LID - 10.1002/dta.1469 [doi]
AB  - Circulating microRNAs (miRNAs) in plasma are being studied for use as biomarkers 
      of specific diseases and as markers of administration of pharmaceutical agents. 
      Administration of recombinant human growth hormone (rhGH) is prohibited by 
      sporting authorities, but it continues to be used by athletes attempting to gain 
      an unfair advantage in athletic competition. Current methods for detection of 
      rhGH use rely on immunoassay technology and are limited by a short time-frame in 
      which detection of rhGH is possible. We hypothesized that administration of rhGH 
      would alter expression of circulating miRNAs and that any changes could be 
      detected. To identify potential miRNA targets, we utilized miRNA microarrays for 
      screening. Confirmatory testing used real-time reverse-transcriptase (RT) 
      quantitative PCR (qPCR) assays of selected miRNAs in 35 plasma samples obtained 
      from (1) individuals with no known pituitary disorders, (2) patients with excess 
      GH production, and (3) patients receiving therapeutic replacement doses of rhGH. 
      We identified and confirmed four miRNAs that were differentially expressed in all 
      individuals using therapeutic replacement doses of rhGH when compared to 
      individuals with naturally high levels of GH and normal controls. This study 
      further develops the hypothesis that circulating miRNAs may be used as biomarkers 
      for detection of doping in sports.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Kelly, Brian N
AU  - Kelly BN
AD  - University of Virginia School of Medicine, Charlottesville, VA, USA.
FAU - Haverstick, Doris M
AU  - Haverstick DM
FAU - Lee, Jae K
AU  - Lee JK
FAU - Thorner, Michael O
AU  - Thorner MO
FAU - Vance, Mary Lee
AU  - Vance ML
FAU - Xin, Wenjun
AU  - Xin W
FAU - Bruns, David E
AU  - Bruns DE
LA  - eng
PT  - Journal Article
DEP - 20130312
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (MicroRNAs)
RN  - 0 (Recombinant Proteins)
RN  - 12629-01-5 (Human Growth Hormone)
SB  - IM
MH  - Doping in Sports
MH  - Human Growth Hormone/*administration & dosage
MH  - Humans
MH  - MicroRNAs/analysis/*blood
MH  - Real-Time Polymerase Chain Reaction
MH  - Recombinant Proteins/administration & dosage
MH  - Substance Abuse Detection/*methods
OTO - NOTNLM
OT  - acromegaly
OT  - circulating nucleic acids
OT  - doping
OT  - growth hormone
OT  - microRNA
OT  - performance enhancement
OT  - sports
EDAT- 2013/03/16 06:00
MHDA- 2014/11/11 06:00
CRDT- 2013/03/16 06:00
PHST- 2012/09/27 00:00 [received]
PHST- 2013/02/04 00:00 [revised]
PHST- 2013/02/05 00:00 [accepted]
PHST- 2013/03/16 06:00 [entrez]
PHST- 2013/03/16 06:00 [pubmed]
PHST- 2014/11/11 06:00 [medline]
AID - 10.1002/dta.1469 [doi]
PST - ppublish
SO  - Drug Test Anal. 2014 Mar;6(3):234-8. doi: 10.1002/dta.1469. Epub 2013 Mar 12.