PMID- 23496784
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130319
LR  - 20140731
IS  - 1753-0059 (Print)
IS  - 1753-0059 (Linking)
VI  - 2
IP  - 12
DP  - 2008 Dec
TI  - Fluorescence in situ hybridization techniques in medical diagnostics.
PG  - 1381-90
LID - 10.1517/17530050802558899 [doi]
AB  - BACKGROUND: Fluorescence in situ hybridization (FISH) has become a 
      well-established method in medical diagnostics. FISH methods complement 
      conventional cytogenetic banding techniques and offer extra clinical 
      applications. FISH is based on the binding of complementary, single-stranded 
      fluorescence-labeled nucleic acid sequences to the fixed and denatured target DNA 
      of metaphases, interphase nuclei or isolated DNA sequences (BACs, 
      oligonucleotides). OBJECTIVE: The intent of this article is to review the 
      development of molecular cytogenetic techniques available at present and to 
      summarize the most efficient and appropriate use of these techniques in medical 
      diagnostics. The technical aspects and most important applications of FISH assays 
      are described. CONCLUSION: FISH is bridging the gap between conventional 
      cytogenetic banding analysis and molecular genetic DNA studies. The use of FISH 
      techniques enhances the correct interpretation of numerical and structural 
      chromosome aberrations.
FAU - Stumm, Markus
AU  - Stumm M
AD  - Centre for Prenatal Diagnosis, Kudamm 199, Berlin 10719, Germany.
FAU - Tonnies, Holger
AU  - Tonnies H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Expert Opin Med Diagn
JT  - Expert opinion on medical diagnostics
JID - 101392201
EDAT- 2008/12/01 00:00
MHDA- 2008/12/01 00:01
CRDT- 2013/03/19 06:00
PHST- 2013/03/19 06:00 [entrez]
PHST- 2008/12/01 00:00 [pubmed]
PHST- 2008/12/01 00:01 [medline]
AID - 10.1517/17530050802558899 [doi]
PST - ppublish
SO  - Expert Opin Med Diagn. 2008 Dec;2(12):1381-90. doi: 10.1517/17530050802558899.