PMID- 23497598
OWN - NLM
STAT- MEDLINE
DCOM- 20130628
LR  - 20211021
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 9
DP  - 2013 Mar 8
TI  - Novel lipoprotein density profiling in healthy dogs of various breeds, healthy 
      Miniature Schnauzers, and Miniature Schnauzers with hyperlipidemia.
PG  - 47
LID - 10.1186/1746-6148-9-47 [doi]
AB  - BACKGROUND: Despite the importance of abnormalities in lipoprotein metabolism in 
      clinical canine medicine, the fact that most previously used methods for 
      lipoprotein profiling are rather laborious and time-consuming has been a major 
      obstacle to the wide clinical application and use of lipoprotein profiling in 
      this species. The aim of the present study was to assess the feasibility of a 
      continuous lipoprotein density profile (CLPDP) generated within a bismuth sodium 
      ethylenediaminetetraacetic acid (NaBiEDTA) density gradient to characterize and 
      compare the lipoprotein profiles of healthy dogs of various breeds, healthy 
      Miniature Schnauzers, and Miniature Schnauzers with primary 
      hypertriacylglycerolemia. A total of 35 healthy dogs of various breeds with serum 
      triacylglycerol (TAG) and cholesterol concentrations within their respective 
      reference intervals were selected for use as a reference population. Thirty-one 
      Miniature Schnauzers with serum TAG and cholesterol concentrations within their 
      respective reference intervals and 31 Miniature Schnauzers with 
      hypertriacylglyceridemia were also included in the study. RESULTS: The results 
      suggest that CLPDP using NaBiEDTA provides unique diagnostic information in 
      addition to measurements of serum TAG and cholesterol concentrations and that it 
      is a useful screening method for dogs with suspected lipoprotein metabolism 
      disorders. Using the detailed and continuous density distribution information 
      provided by the CLPDP, important differences in lipoprotein profiles can be 
      detected even among dogs that have serum TAG and cholesterol concentrations 
      within the reference interval. Miniature Schnauzers with serum TAG and 
      cholesterol concentrations within the reference interval had significantly 
      different lipoprotein profiles than dogs of various other breeds. In addition, it 
      was further established that specific lipoprotein fractions are associated with 
      hypertriacylglyceridemia in Miniature Schnauzers. CONCLUSIONS: The results of the 
      present study suggest that density gradient ultracentrifugation using NaBiEDTA is 
      a useful screening method for the study of lipoprotein profiles in dogs. 
      Therefore, this method could potentially be used for diagnostic purposes for the 
      separation of dogs suspected of having lipoprotein abnormalities from healthy 
      dogs.
FAU - Xenoulis, Panagiotis G
AU  - Xenoulis PG
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 
      4474 TAMU, College Station, TX 77843, USA. pxenoulis@gmail.com
FAU - Cammarata, Paul J
AU  - Cammarata PJ
FAU - Walzem, Rosemary L
AU  - Walzem RL
FAU - Macfarlane, Ronald D
AU  - Macfarlane RD
FAU - Suchodolski, Jan S
AU  - Suchodolski JS
FAU - Steiner, Jorg M
AU  - Steiner JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130308
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Lipoproteins)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Animals
MH  - Cholesterol/blood
MH  - Dog Diseases/*blood
MH  - Dogs/*blood
MH  - Edetic Acid
MH  - Humans
MH  - Hyperlipidemias/blood/*veterinary
MH  - Lipoproteins/*blood
MH  - Male
MH  - Species Specificity
MH  - Triglycerides/blood
PMC - PMC3606259
EDAT- 2013/03/19 06:00
MHDA- 2013/07/03 06:00
PMCR- 2013/03/08
CRDT- 2013/03/19 06:00
PHST- 2012/12/03 00:00 [received]
PHST- 2013/02/26 00:00 [accepted]
PHST- 2013/03/19 06:00 [entrez]
PHST- 2013/03/19 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
PHST- 2013/03/08 00:00 [pmc-release]
AID - 1746-6148-9-47 [pii]
AID - 10.1186/1746-6148-9-47 [doi]
PST - epublish
SO  - BMC Vet Res. 2013 Mar 8;9:47. doi: 10.1186/1746-6148-9-47.