PMID- 23498791
OWN - NLM
STAT- MEDLINE
DCOM- 20130830
LR  - 20151119
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 45
IP  - 2
DP  - 2013 Mar
TI  - Immune tolerance of skin allograft transplantation induced by immature dendritic 
      cells of a third party carrying donor antigens in mice.
PG  - 552-7
LID - S0041-1345(12)01353-X [pii]
LID - 10.1016/j.transproceed.2012.07.161 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the most powerful antigen-presenting cells 
      in the body. Immature DCs (imDCs) can induce transplantation tolerance. In this 
      study, using a mouse model of skin transplantation. We explored the antigen 
      uptake by imDCs, changes in phenotype and function after antigen loading, as well 
      as survival of skin grafts. METHODS: Mononuclear cells from C57BL/6 mice mixed 
      with a tritiated leucine ([(3)H]Leu) antigen supernate were incubated with 
      Kunming mice imDC and mature DCs. We recorded the expressions of surface 
      molecules that were detected using flow cytometry, mixed lymphocyte reactions, 
      mean survival times, and postoperative morphological changes in skin grafts. 
      RESULTS: After the addition of allogeneic antigen supernate, the counts per 
      minute of imDCs were significantly higher than those of mature DCs. The 
      expression rates of I(A)/I(E) and CD80 significantly increased on the cell 
      surface of imDCs. The counts per minute of imDCs in mixed lymphocyte reactions in 
      the presence of allogeneic antigens was significantly higher than those of 
      controls. Comparing mean survival times with controls, skin grafts were 
      significantly longer in the imDCs groups from donors or from a third party 
      carrying donor antigens. CONCLUSIONS: ImDCs display a strong antigen uptake, 
      gradually maturing in terms of phenotype and function after loading. 
      Complementary application of cytotoxic T-lymphocyte-associated antigen 4 
      immunoglobulin blocks the immune response of imDCs. Both imDCs from the third 
      party carrying donor antigens and those from the donor strain can establish 
      antigen-specific immune tolerance to allogeneic skin grafts.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Xin, H
AU  - Xin H
AD  - Burn and Plastic Surgery Department of PLA 181 Hospital, Guilin, Guangxi, China.
FAU - Yang, W
AU  - Yang W
FAU - Wang, Q
AU  - Wang Q
FAU - You, B
AU  - You B
FAU - Tong, Y
AU  - Tong Y
FAU - Peng, Y
AU  - Peng Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Antigens)
RN  - 0 (Immunoconjugates)
RN  - 0 (Immunosuppressive Agents)
RN  - 7D0YB67S97 (Abatacept)
SB  - IM
MH  - Abatacept
MH  - Animals
MH  - Antigens/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/immunology/*transplantation
MH  - Female
MH  - Flow Cytometry
MH  - Graft Rejection/immunology/*prevention & control
MH  - *Graft Survival/drug effects
MH  - Immunoconjugates/pharmacology
MH  - Immunosuppressive Agents/pharmacology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Skin Transplantation/adverse effects/*immunology
MH  - Time Factors
MH  - *Transplantation Tolerance/drug effects
EDAT- 2013/03/19 06:00
MHDA- 2013/08/31 06:00
CRDT- 2013/03/19 06:00
PHST- 2012/01/12 00:00 [received]
PHST- 2012/07/05 00:00 [revised]
PHST- 2012/07/19 00:00 [accepted]
PHST- 2013/03/19 06:00 [entrez]
PHST- 2013/03/19 06:00 [pubmed]
PHST- 2013/08/31 06:00 [medline]
AID - S0041-1345(12)01353-X [pii]
AID - 10.1016/j.transproceed.2012.07.161 [doi]
PST - ppublish
SO  - Transplant Proc. 2013 Mar;45(2):552-7. doi: 10.1016/j.transproceed.2012.07.161.