PMID- 23498811
OWN - NLM
STAT- MEDLINE
DCOM- 20130830
LR  - 20130318
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 45
IP  - 2
DP  - 2013 Mar
TI  - Migration and activation of T cells during development of graft-versus-host 
      disease in a mouse model.
PG  - 713-8
LID - S0041-1345(12)01321-8 [pii]
LID - 10.1016/j.transproceed.2012.12.004 [doi]
AB  - OBJECTIVE: We investigated the pathophysiologic events in graft-versus-host 
      disease (GVHD), a major complication of hematopoietic stem cell transplantation 
      (HSCT). METHODS: The experimental group included BALB/c female mice conditioned 
      with 8.0 GY total body irradiation that were transplanted with allogeneic C57BL/6 
      male bone marrow cells (BMCs) plus CD4(+) T cells and CD8(+) T cells isolated 
      from green fluorescent protein transgenic (eGFP-Tg) C57BL/6 male mice by the 
      immunomagnetic beads negative sorting method. The control group was transplanted 
      only with C57BL/6 male BMCs. RESULTS: GVHD clinical manifestations were present 
      in the BMCs plus T-cell-transplanted group, but not the BMCs alone group. eGFP(+) 
      T cells were observed in recipient organs, including the liver, spleen, 
      intestine, skin, lungs, tongue, kidneys and even the brain. Donor eGFP(+) T cells 
      were significantly increased in liver and spleen before day +4 (P < .05); but 
      decreased in the spleen while still increased in the liver after day +4 (P < 
      .05). CD25 expression of donor eGFP(+) T cells in the liver and spleen, and 
      interleukin (IL)-2 levels in the peripheral blood was significantly increased 
      before day +4 (P < .05), but decreased after day +4 (P < .05). CONCLUSION: These 
      data support the donor T-cell migration hypothesis that accompanied by expression 
      of CD25 and IL-2, during the development of GVHD donor T cells migrate to 
      lymphoid organs, such as the spleen, after activation migrating to GVHD target 
      organs to induce GVHD damage.
CI  - Crown Copyright (c) 2013. Published by Elsevier Inc. All rights reserved.
FAU - Wen, H-S
AU  - Wen HS
AD  - Oncology and Hematology Department, Ningbo Development Zone Center Hospital, 
      Ningbo, PR China. WHSJL@163.com
FAU - Wang, J-M
AU  - Wang JM
FAU - Zhou, H
AU  - Zhou H
FAU - Gong, S-I
AU  - Gong SI
FAU - Gao, L
AU  - Gao L
FAU - Wu, Y
AU  - Wu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Il2ra protein, mouse)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism/transplantation
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism/transplantation
MH  - *Chemotaxis, Leukocyte
MH  - Disease Models, Animal
MH  - Female
MH  - Graft vs Host Disease/*immunology/metabolism/pathology
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Hematopoietic Stem Cell Transplantation/adverse effects
MH  - Immunomagnetic Separation
MH  - Interleukin-2/metabolism
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - *Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Time Factors
MH  - Whole-Body Irradiation
EDAT- 2013/03/19 06:00
MHDA- 2013/08/31 06:00
CRDT- 2013/03/19 06:00
PHST- 2012/08/20 00:00 [received]
PHST- 2012/11/04 00:00 [revised]
PHST- 2012/12/03 00:00 [accepted]
PHST- 2013/03/19 06:00 [entrez]
PHST- 2013/03/19 06:00 [pubmed]
PHST- 2013/08/31 06:00 [medline]
AID - S0041-1345(12)01321-8 [pii]
AID - 10.1016/j.transproceed.2012.12.004 [doi]
PST - ppublish
SO  - Transplant Proc. 2013 Mar;45(2):713-8. doi: 10.1016/j.transproceed.2012.12.004.