PMID- 23499541
OWN - NLM
STAT- MEDLINE
DCOM- 20140206
LR  - 20220408
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 197
IP  - 2
DP  - 2013 Aug
TI  - Comparison of different vaccination schedules for sustaining the immune response 
      against porcine reproductive and respiratory syndrome virus.
PG  - 438-44
LID - S1090-0233(13)00070-1 [pii]
LID - 10.1016/j.tvjl.2013.02.008 [doi]
AB  - In order to better understand how immunization against porcine reproductive and 
      respiratory syndrome virus (PRRSV) can be improved using commercial vaccines, 
      different strategies of immunization were applied in the field using an 
      inactivated vaccine (INV), a modified live vaccine (MLV) or a combination of the 
      two and the responses compared. In experiment 1 (E1), 21 piglets were distributed 
      in three groups. Group A was vaccinated with a commercial INV at 2.5, 3.5 and 6.5 
      months old; group B pigs received the INV at 1.5, 2.5, 5.5 and 6.5 months old, 
      while pigs in group C were kept as unvaccinated controls. At 7.5 months of age 
      all pigs were challenged with PRRSV and followed for 21 days. In experiment 2 
      (E2), 32 piglets were distributed evenly in four groups. Groups A, B and C were 
      vaccinated with a commercial MLV at 1.5 months old, while group D pigs were kept 
      as controls. At 4.5 months old, groups A and C received the INV while B received 
      a second MLV, 1 month later group C pigs received a third INV. At 6.5 months old 
      all pigs were challenged as in E1. In both experiments, total antibodies, 
      neutralizing antibodies (NA) and cell-mediated immunity (CMI) were evaluated, and 
      viraemia was determined after challenge. In E1, immunization with an INV induced 
      high interferon-gamma responses after the second and subsequent vaccinations. 
      Development of NA after challenge was faster in INV vaccinated pigs compared to 
      unvaccinated controls. In E2, re-vaccination with INV induced NA responses 
      similar to re-vaccination with MLV; however, a significant increase in NA titres 
      after challenge was only detected in group C pigs. The use of combined protocols 
      (MLV+INV) was superior to the use of MLV alone in inducing cell mediated 
      immunity. In conclusion, the highest immune responses against PRRSV after a 
      single shot were achieved with MLV; after that, INV re-vaccination should be 
      considered as the best strategy to induce significant boosters.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Diaz, I
AU  - Diaz I
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. ivan.diaz@cresa.uab.es
FAU - Gimeno, M
AU  - Gimeno M
FAU - Callen, A
AU  - Callen A
FAU - Pujols, J
AU  - Pujols J
FAU - Lopez, S
AU  - Lopez S
FAU - Charreyre, C
AU  - Charreyre C
FAU - Joisel, F
AU  - Joisel F
FAU - Mateu, E
AU  - Mateu E
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20130315
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - *Immunization Schedule
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - *Vaccination
MH  - Viral Vaccines/*immunology
OTO - NOTNLM
OT  - Cell-mediated immunity
OT  - Neutralizing antibodies
OT  - Porcine reproductive and respiratory syndrome
OT  - Vaccination
EDAT- 2013/03/19 06:00
MHDA- 2014/02/07 06:00
CRDT- 2013/03/19 06:00
PHST- 2012/05/23 00:00 [received]
PHST- 2013/01/11 00:00 [revised]
PHST- 2013/02/07 00:00 [accepted]
PHST- 2013/03/19 06:00 [entrez]
PHST- 2013/03/19 06:00 [pubmed]
PHST- 2014/02/07 06:00 [medline]
AID - S1090-0233(13)00070-1 [pii]
AID - 10.1016/j.tvjl.2013.02.008 [doi]
PST - ppublish
SO  - Vet J. 2013 Aug;197(2):438-44. doi: 10.1016/j.tvjl.2013.02.008. Epub 2013 Mar 15.