PMID- 23500835
OWN - NLM
STAT- MEDLINE
DCOM- 20140207
LR  - 20130517
IS  - 1873-1686 (Electronic)
IS  - 0167-0115 (Linking)
VI  - 184
DP  - 2013 Jun 10
TI  - Antipyretic effect of central [Pyr1]apelin13 on LPS-induced fever in the rat.
PG  - 6-13
LID - S0167-0115(13)00047-5 [pii]
LID - 10.1016/j.regpep.2013.03.018 [doi]
AB  - Intracerebroventricular (i.c.v.) injections of apelins have been shown to 
      modulate the central control of cardiovascular function, as well as the 
      homeostasis of fluid and salt balance, and to some extent also body core 
      temperature. Here, we investigated the effects of i.c.v. administration of 
      [Pyr(1)]apelin13 (PyrAp13; 20nmol) dissolved in artificial cerebrospinal fluid 
      (aCSF), as compared to aCSF alone, on fever and sickness behavior elicited in 
      rats by intraperitoneal injection of bacterial lipopolysaccharide (LPS, 100 
      mug/kg). Injections of LPS induced a short phase of hypothermia followed by a 
      biphasic fever, depression of motor activity, anorexia and adipsia. I.c.v. 
      injections of PyrAp13 without systemic LPS application slightly augmented motor 
      activity at statistically unaltered core temperature. In combination with LPS, 
      central administration of PyrAp13 significantly reduced fever during the time 
      period of 3-9h after injection, but did not significantly attenuate anorexia and 
      adipsia, and had no effect on LPS-induced lethargy. Rats injected with PyrAp13 
      along with LPS showed a reduced level of LPS-induced circulating tumor necrosis 
      factor-alpha (TNF-alpha). Primary neuroglial cultures established from the hypothalamic 
      paraventricular nucleus (PVN) and the median preoptic nucleus (MnPO), brain sites 
      being of major importance for central thermoregulation and also expressing the 
      apelin receptor, were incubated with medium alone, medium containing LPS (100 
      mug/ml) or LPS plus PyrAp13 (10(-6) mol/L). Ninety minutes after start of the 
      incubation, LPS alone but not LPS in combination with PyrAp13 (10(-6) mol/L) 
      caused a significant elevation of TNF-alpha in the supernatants. The novel 
      observation that PyrAp13 represents a centrally acting endogenous antipyretic 
      peptide is discussed in relation to its capacity to modulate peripheral and 
      central formation of TNF-alpha.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Hatzelmann, Thomas
AU  - Hatzelmann T
AD  - Institut fur Veterinar-Physiologie und -Biochemie, Justus-Liebig-Universitat 
      Giessen, Giessen, Germany.
FAU - Harden, Lois M
AU  - Harden LM
FAU - Roth, Joachim
AU  - Roth J
FAU - Gerstberger, Rudiger
AU  - Gerstberger R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130314
PL  - Netherlands
TA  - Regul Pept
JT  - Regulatory peptides
JID - 8100479
RN  - 0 (Antipyretics)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (apelin 13, Pyr(1)-)
SB  - IM
MH  - Animals
MH  - Antipyretics/*pharmacology/therapeutic use
MH  - Fever/*drug therapy/metabolism/physiopathology
MH  - Injections, Intraperitoneal
MH  - Intercellular Signaling Peptides and Proteins/*pharmacology/therapeutic use
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Paraventricular Hypothalamic Nucleus/metabolism
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2013/03/19 06:00
MHDA- 2014/02/08 06:00
CRDT- 2013/03/19 06:00
PHST- 2012/08/20 00:00 [received]
PHST- 2013/01/15 00:00 [revised]
PHST- 2013/03/03 00:00 [accepted]
PHST- 2013/03/19 06:00 [entrez]
PHST- 2013/03/19 06:00 [pubmed]
PHST- 2014/02/08 06:00 [medline]
AID - S0167-0115(13)00047-5 [pii]
AID - 10.1016/j.regpep.2013.03.018 [doi]
PST - ppublish
SO  - Regul Pept. 2013 Jun 10;184:6-13. doi: 10.1016/j.regpep.2013.03.018. Epub 2013 
      Mar 14.