PMID- 23504611
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240322
IS  - 1616-301X (Print)
IS  - 1616-3028 (Electronic)
IS  - 1616-301X (Linking)
VI  - 19
IP  - 19
DP  - 2009 Oct 9
TI  - Affinity-based Protein Surface Pattern Formation by Ligand Self-Selection from 
      Mixed Protein Solutions.
PG  - 3046-3055
AB  - Photolithographically prepared surface patterns of two affinity ligands (biotin 
      and chloroalkane) specific for two proteins (streptavidin and HaloTag(R), 
      respectively) are used to spontaneously form high-fidelity surface patterns of 
      the two proteins from their mixed solution. High affinity protein-surface 
      self-selection onto patterned ligands on surfaces exhibiting low non-specific 
      adsorption rapidly yields the patterned protein surfaces. Fluorescence images 
      after protein immobilization show high specificity of the target proteins to 
      their respective surface patterned ligands. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) imaging further supports the chemical specificity of 
      streptavidin and HaloTag(R) for their surface patterned ligands from mixed protein 
      solutions. However, ToF-SIMS did detect some non-specific adsorption of bovine 
      serum albumin, a masking protein present in excess in the adsorbing solutions, on 
      the patterned surfaces. Protein amino acid composition, surface coverage, density 
      and orientation are important parameters that determine the relative ToF-SIMS 
      fragmentation pattern yields. ToF-SIMS amino acid-derived ion fragment yields 
      summed to produce surface images can reliably determine which patterned surface 
      regions contain bound proteins, but do not readily discriminate between different 
      co-planar protein regions. Principal component analysis (PCA) of these ToF-SIMS 
      data, however, improves discrimination of ions specific to each protein, 
      facilitating surface pattern discrimination and contrast.
FAU - Grainger, David W
AU  - Grainger DW
AD  - Departments of Pharmaceutics and Pharmaceutical Chemistry, and Bioengineering, 
      University of Utah, Salt Lake City, UT 84112-5820 (USA).
FAU - Castner, David G
AU  - Castner DG
FAU - Dubey, Manish
AU  - Dubey M
FAU - Emoto, Kazunori
AU  - Emoto K
FAU - Takahashi, Hironobu
AU  - Takahashi H
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - R01 EB001473/EB/NIBIB NIH HHS/United States
PT  - Journal Article
DEP - 20090810
PL  - Germany
TA  - Adv Funct Mater
JT  - Advanced functional materials
JID - 101190390
PMC - PMC3597123
MID - NIHMS430275
OTO - NOTNLM
OT  - ToF-SIMS
OT  - patterned proteins
OT  - photolithography
OT  - poly(ethylene glycol)
OT  - protein imaging
EDAT- 2009/10/09 00:00
MHDA- 2009/10/09 00:01
PMCR- 2013/03/14
CRDT- 2013/03/19 06:00
PHST- 2013/03/19 06:00 [entrez]
PHST- 2009/10/09 00:00 [pubmed]
PHST- 2009/10/09 00:01 [medline]
PHST- 2013/03/14 00:00 [pmc-release]
AID - 10.1002/adfm.200900809 [doi]
PST - ppublish
SO  - Adv Funct Mater. 2009 Oct 9;19(19):3046-3055. doi: 10.1002/adfm.200900809. Epub 
      2009 Aug 10.