PMID- 23507851
OWN - NLM
STAT- MEDLINE
DCOM- 20130909
LR  - 20211021
IS  - 1095-9114 (Electronic)
IS  - 0895-3996 (Print)
IS  - 0895-3996 (Linking)
VI  - 21
IP  - 1
DP  - 2013
TI  - DOT corrected fluorescence molecular tomography using targeted contrast agents 
      for small animal tumor imaging.
PG  - 43-52
LID - 10.3233/XST-130365 [doi]
AB  - PURPOSE: To demonstrate diffuse optical tomography (DOT) corrected fluorescence 
      molecular tomography (FMT) for quantitatively imaging tumor-targeted contrast 
      agents in a 4T1 mouse mammary tumor model. PROCEDURES: In the first set of 
      experiments, we validated our DOT corrected FMT method using subcutaneously 
      injected 4T1 cells pre-labeled with a near-infrared (NIR) Cy 5.5 dye labeled 
      recombinant amino-terminal fragment (ATF) of the receptor binding domain of 
      urokinase plasminogen activator (uPA), which binds to uPA receptor (uPAR) that is 
      highly expressed in breast cancer tissues. Next, we apply the DOT corrected FMT 
      method to quantitatively evaluate the ability of sensitive tumor imaging after 
      systemic delivery of new uPAR-targeted optical imaging probes in the mice bearing 
      4T1 mammary tumors. These uPAR-targeted optical imaging probes are ATF peptides 
      labeled with a newly developed NIR-830 dye being conjugated to magnetic iron 
      oxide nanoparticles (IONPs). RESULTS: Our results have shown that DOT corrected 
      FMT can accurately quantify and localize the injected imaging probe labeled 4T1 
      cells. Following systemic delivery of the targeted imaging nanoprobes into the 
      mice bearing orthotopic mammary tumors, specific accumulation of the imaging 
      probes in the orthotopic mammary tumors was detected in the mice that received 
      uPAR targeted NIR-830-ATF-IONP probes but not in the mice injected with 
      non-targeted NIR-830-mouse serum albumin (MSA)-IONPs. Additionally, DOT corrected 
      FMT also enables the detection of both locally recurrent tumor and lung 
      metastasis in the mammary tumor model 72 hrs after systemic administration of the 
      uPAR-targeted NIR-830-labeled ATF peptide imaging probes. CONCLUSIONS: DOT 
      corrected FMT and uPAR-targeted optical imaging probes have great potential for 
      detection of breast cancer, recurrent tumor and metastasis in small animals.
FAU - Tan, Yiyong
AU  - Tan Y
AD  - J. Crayton Pruitt Family Department of Biomedical Engineering, University of 
      Florida, Gainesville, FL 32611-6131, USA.
FAU - Cao, Zehong
AU  - Cao Z
FAU - Sajja, Hari Krishna
AU  - Sajja HK
FAU - Lipowska, Malgorzata
AU  - Lipowska M
FAU - Wang, Y Andrew
AU  - Wang YA
FAU - Yang, Lily
AU  - Yang L
FAU - Jiang, Huabei
AU  - Jiang H
LA  - eng
GR  - R01 CA133722/CA/NCI NIH HHS/United States
GR  - R01 CA154129/CA/NCI NIH HHS/United States
GR  - R01 CA154846/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Netherlands
TA  - J Xray Sci Technol
JT  - Journal of X-ray science and technology
JID - 9000080
RN  - 0 (CY5.5 cyanine dye)
RN  - 0 (Carbocyanines)
RN  - 0 (Contrast Media)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Carbocyanines/chemistry/pharmacokinetics
MH  - Cell Line, Tumor
MH  - Contrast Media/chemistry/*pharmacokinetics
MH  - Female
MH  - Fluorescent Dyes/chemistry/pharmacokinetics
MH  - Image Processing, Computer-Assisted
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasms, Experimental/metabolism/*pathology
MH  - Optical Imaging/*methods
MH  - Receptors, Urokinase Plasminogen Activator/metabolism
MH  - Tomography, Optical/*methods
MH  - Urokinase-Type Plasminogen Activator/chemistry/metabolism/pharmacokinetics
MH  - Whole Body Imaging/methods
PMC - PMC3785613
MID - NIHMS496930
EDAT- 2013/03/20 06:00
MHDA- 2013/09/10 06:00
PMCR- 2014/01/01
CRDT- 2013/03/20 06:00
PHST- 2013/03/20 06:00 [entrez]
PHST- 2013/03/20 06:00 [pubmed]
PHST- 2013/09/10 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 30605P6420828713 [pii]
AID - 10.3233/XST-130365 [doi]
PST - ppublish
SO  - J Xray Sci Technol. 2013;21(1):43-52. doi: 10.3233/XST-130365.