PMID- 23508090
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130320
LR  - 20211021
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 13
IP  - 4
DP  - 2012 Winter
TI  - Evaluation of Bcl-2 Family Gene Expression in Hippocampus of 3, 
      4-methylenedioxymethamphetamine Treated Rats.
PG  - 275-80
AB  - OBJECTIVE: 3,4-methylenedioxymethamphetamine (MDMA) is an illicit, recreational 
      drug that causes cellular death and neurotoxicity. This study evaluates the 
      effects of different doses of MDMA on the expression of apoptosis-related 
      proteins and genes in the hippocampus of adult rats. MATERIALS AND METHODS: In 
      this expremental study,a total of 20 male Sprague Dawley rats (200-250 g ) were 
      treated with MDMA (0, 5, 10, 20 mg/kg i.p. twice daily) for 7 days. Seven days 
      after the last administration of MDMA, the rats were killed. Bax and Bcl-2 genes 
      in addition to protein expressions were detected by western blot and reverse 
      transcriptionpolymerase chain reaction (RT-PCR).Results were analyzed using 
      one-way ANOVA and p</=0.05 was considered statistically significant. RESULTS: Our 
      results showed that MDMA caused dose dependent up-regulation of Bax and 
      down-regulation of Bcl-2 in the hippocampus. There was a significant alteration 
      in bcl-2 and bax genes density. CONCLUSION: Changes in apoptosis-related proteins 
      and respective genes relating to Bax and Bcl-2 might be involved in the molecular 
      mechanism of MDMA-induced apoptosis.
FAU - Soleimani Asl, Sara
AU  - Soleimani Asl S
AD  - 1. Department of Anatomical Sciences, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Farhadi, Mohammad Hassan
AU  - Farhadi MH
FAU - Moosavizadeh, Kazem
AU  - Moosavizadeh K
FAU - Samadi Kuchak Saraei, Ali
AU  - Samadi Kuchak Saraei A
FAU - Soleimani, Mansoure
AU  - Soleimani M
FAU - Jamei, Seid Behnameldin
AU  - Jamei SB
FAU - Joghataei, Mohammad Taghi
AU  - Joghataei MT
FAU - Samzadeh-Kermani, Alireza
AU  - Samzadeh-Kermani A
FAU - Hashemi-Nasl, Hamed
AU  - Hashemi-Nasl H
FAU - Mehdizadeh, Mehdi
AU  - Mehdizadeh M
LA  - eng
PT  - Journal Article
DEP - 20111222
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC3584478
OTO - NOTNLM
OT  - 3, 4-methylendioxymethamphetamine (MDMA)
OT  - Apoptosis
OT  - Bax
OT  - Bcl-2
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/12/01
CRDT- 2013/03/20 06:00
PHST- 2010/04/05 00:00 [received]
PHST- 2011/09/20 00:00 [accepted]
PHST- 2013/03/20 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
PST - ppublish
SO  - Cell J. 2012 Winter;13(4):275-80. Epub 2011 Dec 22.