PMID- 23508550 OWN - NLM STAT- MEDLINE DCOM- 20130930 LR - 20130726 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 158 IP - 8 DP - 2013 Aug TI - Complete nucleotide sequence of a new isolate of passion fruit woodiness virus from Western Australia. PG - 1821-4 LID - 10.1007/s00705-013-1670-2 [doi] AB - We determined the complete genome sequence of the passion fruit woodiness virus Gld-1 isolate (PWV-Gld-1) from Australia and compared it with that of PWV-MU-2, another Australian isolate of PWV. The genomes shared high sequence identity in both the complete nucleotide sequence and the ORF amino acid sequence. All of the cleavage sites of each protein were identical to those of MU-2, and the sequence identity for the individual proteins ranged from 97.2 % to 100.0 %. However, the 5' untranslated region (5'UTR) of the Gld-1 isolate shared only 46.8 % sequence identity with that of PWV-MU-2 and was 177 nucleotides shorter. Re-sequencing of the 5'UTR of MU-2 revealed that the 5' end of the original sequence includes an artifact generated by deep sequencing. FAU - Fukumoto, Tomohiro AU - Fukumoto T AD - Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan. FAU - Nakamura, Masayuki AU - Nakamura M FAU - Wylie, Stephen J AU - Wylie SJ FAU - Chiaki, Yuya AU - Chiaki Y FAU - Iwai, Hisashi AU - Iwai H LA - eng SI - GENBANK/HQ122652 PT - Journal Article DEP - 20130319 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (RNA, Viral) SB - IM MH - Cluster Analysis MH - *Genome, Viral MH - Molecular Sequence Data MH - Phylogeny MH - Plant Diseases/virology MH - Potyvirus/*genetics/isolation & purification MH - RNA, Viral/*genetics MH - *Sequence Analysis, DNA MH - Sequence Homology MH - Western Australia EDAT- 2013/03/20 06:00 MHDA- 2013/10/01 06:00 CRDT- 2013/03/20 06:00 PHST- 2012/12/26 00:00 [received] PHST- 2013/02/07 00:00 [accepted] PHST- 2013/03/20 06:00 [entrez] PHST- 2013/03/20 06:00 [pubmed] PHST- 2013/10/01 06:00 [medline] AID - 10.1007/s00705-013-1670-2 [doi] PST - ppublish SO - Arch Virol. 2013 Aug;158(8):1821-4. doi: 10.1007/s00705-013-1670-2. Epub 2013 Mar 19.