PMID- 23508811
OWN - NLM
STAT- MEDLINE
DCOM- 20140519
LR  - 20231213
IS  - 1662-5110 (Print)
IS  - 1662-5110 (Electronic)
IS  - 1662-5110 (Linking)
VI  - 7
DP  - 2013
TI  - Functional imaging in the zebrafish retinotectal system using RGECO.
PG  - 34
LID - 10.3389/fncir.2013.00034 [doi]
LID - 34
AB  - Genetically encoded calcium indicators (GECIs) allow repeated, non-invasive 
      measurements of neural activity in defined populations of neurons, but until 
      recently GECIs based on single fluorescent proteins have been limited to the 
      green region of the color spectrum. Recent efforts in protein engineering have 
      expanded the color palette of GECIs. One of these new GECIs, the red RGECO, is 
      spectrally separate from the traditional GFP-based sensors such as GCaMP, and 
      therefore opens the way for simultaneous, multicolor imaging of neural activity. 
      While RGECO has been shown to report spontaneous calcium fluctuations in neurons, 
      the precise relationship of RGECO signal to evoked-neural activity is not known. 
      Measurements of neural activity using RGECO in vivo have also not been reported. 
      Using dissociated hippocampal neurons we performed a systematic analysis of two 
      forms of RGECO- a cytosolic form and a presynaptically localized form generated 
      by fusion of RGECO to the presynaptic protein, synaptophysin (SyRGECO). We find 
      that RGECO and GCaMP3 are comparable in terms of dynamic range, signal-to-noise 
      ratios and kinetics but that RGECO is a more reliable reporter of single action 
      potentials. In terms of performance SyGCaMP3 and SyRGECO are comparable, and both 
      are more sensitive reporters of activity than the cytosolic form of each probe. 
      Using the zebrafish retinotectal system we show that SyRGECO and RGECO are can 
      report neural activity in vivo and that RGECO expression permits detailed 
      structural analysis of neuronal arbors. We have exploited these attributes to 
      provide a morphological and functional description of tectal cells selective for 
      motion along the vertical axis. These results open up the possibility of using 
      zebrafish to functionally image genetically defined pre- and postsynaptic circuit 
      components, separable by color, which will be a powerful approach to studying 
      neural interactions in the brain.
FAU - Walker, Alison S
AU  - Walker AS
AD  - MRC Centre for Developmental Neurobiology, King's College London London, UK.
FAU - Burrone, Juan
AU  - Burrone J
FAU - Meyer, Martin P
AU  - Meyer MP
LA  - eng
GR  - 095589/WT_/Wellcome Trust/United Kingdom
GR  - G0901307/MRC_/Medical Research Council/United Kingdom
GR  - G0901899/MRC_/Medical Research Council/United Kingdom
GR  - G1100162/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130306
PL  - Switzerland
TA  - Front Neural Circuits
JT  - Frontiers in neural circuits
JID - 101477940
RN  - 0 (Luminescent Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cells, Cultured
MH  - Hippocampus/chemistry/physiology
MH  - Luminescent Proteins/*analysis
MH  - Microinjections/methods
MH  - Molecular Imaging/*methods
MH  - Photic Stimulation/methods
MH  - Retina/*chemistry/*physiology
MH  - Superior Colliculi/*chemistry/*physiology
MH  - Zebrafish
MH  - Red Fluorescent Protein
PMC - PMC3589694
OTO - NOTNLM
OT  - RGECO
OT  - SyRGECO
OT  - calcium indicator
OT  - direction selectivity
OT  - in vivo imaging
OT  - orientation selectivity
OT  - zebrafish
EDAT- 2013/03/20 06:00
MHDA- 2013/03/20 06:01
PMCR- 2013/01/01
CRDT- 2013/03/20 06:00
PHST- 2013/01/17 00:00 [received]
PHST- 2013/02/15 00:00 [accepted]
PHST- 2013/03/20 06:00 [entrez]
PHST- 2013/03/20 06:00 [pubmed]
PHST- 2013/03/20 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.3389/fncir.2013.00034 [doi]
PST - epublish
SO  - Front Neural Circuits. 2013 Mar 6;7:34. doi: 10.3389/fncir.2013.00034. 
      eCollection 2013.