PMID- 23509890
OWN - NLM
STAT- MEDLINE
DCOM- 20140211
LR  - 20171116
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 42
IP  - 4
DP  - 2013
TI  - Enhanced maturation and function of dendritic cells using hydrogel coated plate 
      and antigen electroporation.
PG  - 341-55
LID - 10.3109/08820139.2012.757234 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that can be matured in 
      vitro from immature dendritic cells (iDCs) in the presence of several biological 
      agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are 
      necessary and achieved using various protocols, such as lipofection, passive 
      pulse or electroporation. However, these DCs maturation protocols may cause with 
      a significant loss of cells because of cellular attachment and spreading during 
      culturing. Some biomaterials that influence adhesion and development of cells 
      have been used in cell culture techniques, and it was thought that they might be 
      applied on the culture of DCs. In this study, we used polyHEMA, which is a 
      hydrogel coating biomaterial that prevents DCs from adherence, and investigated 
      whether hydrogel coating affects the maturation of iDCs. The efficiency in the 
      generation of mDCs was improved through hydrogel coating procedure and a 
      dendritic cell maturation marker, CD83, was significantly increased in 
      hydrogel-coated culture condition. The antigen-loaded mDCs from electroporation 
      were further expressed the CD83. The mDCs generated in the hydrogel-coated 
      culture condition showed more, longer and thicker dendrites, and produced more 
      amounts of cytokines such as IL-12 and IFN-gamma. Therefore, it was suggested that 
      the hydrogel-coated culture condition could improve function of mDCs. Cheol-Hun 
      Son and Jae-Ho Bae contributed equally to this work.
FAU - Son, Cheol-Hun
AU  - Son CH
AD  - Department of Research Center, Dongnam Institute of Radiological & Medical 
      Sciences, Busan 619-953, South Korea.
FAU - Bae, Jae-Ho
AU  - Bae JH
FAU - Shin, Dong Yeok
AU  - Shin DY
FAU - Lee, Hong-Rae
AU  - Lee HR
FAU - Choi, Yung Hyun
AU  - Choi YH
FAU - Yang, Kwangmo
AU  - Yang K
FAU - Park, You-Soo
AU  - Park YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130319
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Antibodies)
RN  - 0 (Antigens)
RN  - 187348-17-0 (Interleukin-12)
RN  - 25249-16-5 (Polyhydroxyethyl Methacrylate)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Antigens/chemistry
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Electroporation
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate
MH  - Interferon-gamma/immunology
MH  - Interleukin-12/immunology
MH  - K562 Cells
MH  - Polyhydroxyethyl Methacrylate/*pharmacology
EDAT- 2013/03/21 06:00
MHDA- 2014/02/12 06:00
CRDT- 2013/03/21 06:00
PHST- 2013/03/21 06:00 [entrez]
PHST- 2013/03/21 06:00 [pubmed]
PHST- 2014/02/12 06:00 [medline]
AID - 10.3109/08820139.2012.757234 [doi]
PST - ppublish
SO  - Immunol Invest. 2013;42(4):341-55. doi: 10.3109/08820139.2012.757234. Epub 2013 
      Mar 19.