PMID- 23510473
OWN - NLM
STAT- MEDLINE
DCOM- 20130506
LR  - 20231011
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 46
IP  - 2
DP  - 2013 Apr
TI  - Genista sessilifolia DC. extracts induce apoptosis across a range of cancer cell 
      lines.
PG  - 183-92
LID - 10.1111/cpr.12022 [doi]
AB  - OBJECTIVES: Restorative properties of medicinal plants such as Genista 
      sessilifolia DC. have often been suggested to occur, in epidemiological studies. 
      However, full characterization of effective principles responsible for this 
      action has never previously been performed. Here, we have characterized G. 
      sessilifolia's anti-cancer effects and identified the chemical components 
      involved in this anti-tumour action. MATERIALS AND METHODS: Cell cycle, 
      apoptosis, necrosis, differentiation analyses, high-performance liquid 
      chromatography, western blotting, RNA extraction, real-time PCR and primers have 
      all been observed/used in the study. RESULTS: We report that G. sessilifolia 
      methanol extract has anti-cancer activity on solid and haematological cancer 
      cells. G. sessilifolia extract's anti-proliferative action is closely bound to 
      induction of apoptosis, whereas differentiation is only weakly modulated. 
      Analysis of G. sessilifolia extract, by high-performance liquid chromatography, 
      identifies fraction 18-22 as the pertinent component for induction of apoptosis, 
      whereas fractions 11-13 and 27-30 both seem to contribute to differentiation. G. 
      sessilifolia extract induces apoptosis mediated by caspase activation and p21, 
      Rb, p53, Bcl2-associated agonist of cell death (BAD), tumour necrosis factor 
      receptor super-family, member 10 (TRAIL) overexpression and death receptor 5 
      (DR5). Accordingly, fraction 18-22 inducing apoptosis was able to induce TRAIL. 
      CONCLUSIONS: Our results indicate that G. sessilifolia extract and its fraction 
      18-22 containing genistin and isoprunetin, were able to induce anti-cancer 
      effects supporting the hypothesis of a pro-apoptotic intrinsic content of this 
      natural medicinal plant.
CI  - (c) 2013 Blackwell Publishing Ltd.
FAU - Bontempo, P
AU  - Bontempo P
AD  - Department of General Pathology, Seconda Universita degli Studi di Napoli, Vico 
      Luigi de Crecchio 7, Naples, 80138, Italy.
FAU - Rigano, D
AU  - Rigano D
FAU - Doto, A
AU  - Doto A
FAU - Formisano, C
AU  - Formisano C
FAU - Conte, M
AU  - Conte M
FAU - Nebbioso, A
AU  - Nebbioso A
FAU - Carafa, V
AU  - Carafa V
FAU - Caserta, G
AU  - Caserta G
FAU - Sica, V
AU  - Sica V
FAU - Molinari, A M
AU  - Molinari AM
FAU - Altucci, L
AU  - Altucci L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (BAD protein, human)
RN  - 0 (Isoflavones)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFSF10 protein, human)
RN  - 0 (bcl-Associated Death Protein)
RN  - 1POG3SCN5T (genistin)
RN  - DH2M523P0H (Genistein)
RN  - EC 3.4.22.- (CASP8 protein, human)
RN  - EC 3.4.22.- (Caspase 8)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/chemistry/*pharmacology
MH  - *Apoptosis
MH  - Caspase 8/chemistry/genetics
MH  - Cell Cycle
MH  - Cell Differentiation
MH  - Cell Proliferation/drug effects
MH  - Chemical Fractionation/methods
MH  - Chromatography, High Pressure Liquid
MH  - Enzyme Activation
MH  - Flow Cytometry
MH  - Genista/*chemistry
MH  - Genistein/chemistry/isolation & purification/pharmacology
MH  - Granulocytes/drug effects/pathology
MH  - HeLa Cells
MH  - Humans
MH  - Isoflavones/chemistry/isolation & purification/pharmacology
MH  - MCF-7 Cells
MH  - Methanol/chemistry
MH  - Plant Components, Aerial/chemistry
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/chemistry/genetics
MH  - TNF-Related Apoptosis-Inducing Ligand/genetics/metabolism
MH  - U937 Cells
MH  - bcl-Associated Death Protein/genetics/metabolism
PMC - PMC6495920
EDAT- 2013/03/21 06:00
MHDA- 2013/05/07 06:00
PMCR- 2013/03/19
CRDT- 2013/03/21 06:00
PHST- 2012/09/17 00:00 [received]
PHST- 2012/11/30 00:00 [accepted]
PHST- 2013/03/21 06:00 [entrez]
PHST- 2013/03/21 06:00 [pubmed]
PHST- 2013/05/07 06:00 [medline]
PHST- 2013/03/19 00:00 [pmc-release]
AID - CPR12022 [pii]
AID - 10.1111/cpr.12022 [doi]
PST - ppublish
SO  - Cell Prolif. 2013 Apr;46(2):183-92. doi: 10.1111/cpr.12022.