PMID- 23510960
OWN - NLM
STAT- MEDLINE
DCOM- 20130826
LR  - 20151119
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1286
DP  - 2013 Apr 19
TI  - Magnetic solid-phase extraction based on magnetic multi-walled carbon nanotubes 
      for the determination of phthalate monoesters in urine samples.
PG  - 22-8
LID - S0021-9673(13)00377-4 [pii]
LID - 10.1016/j.chroma.2013.02.070 [doi]
AB  - In this study, magnetic carbon nanotubes (MCNTs) were prepared by assembling 
      magnetic nanoparticles onto the acid-treated multiwall carbon nanotubes (MWCNTs). 
      Due to their excellent adsorption capability, the MCNTs were used as adsorbent of 
      magnetic solid-phase extraction (MSPE) to extract phthalate monoesters (PMEs), 
      the main biomarkers of phthalate exposure, from human urine. By coupling MSPE 
      with gas chromatography-mass spectrometry (GC-MS), a reliable, sensitive and 
      cost-effective method for the simultaneous determination of five main PMEs 
      including monomethyl phthalate (MMP), monoethyl phthalate (MEP), mono n-butyl 
      phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP) and monobenzyl phthalate 
      (MBzP) was developed. The factors that could influence the extraction, including 
      the amount of magnetic nanoparticles, pH of sample solution, extraction and 
      desorption time, the amount of salt addition, the type and volume of desorption 
      solvent were investigated in detail. Under optimized conditions, the LODs and 
      LOQs achieved were in the range of 0.025-0.050 and 0.125-0.250 ng mL(-1) 
      respectively. And calibration curves were linear (r(2)>/=0.992) over the 
      concentration ranges from 0.250 to 250 ng mL(-1). In addition, a satisfying 
      reproducibility was achieved by evaluating the intra- and inter-day precisions 
      with relative standard deviations (RSDs) less than 11.2% and 11.4%, respectively. 
      The recoveries of the five PMEs ranged from 92.6% to 98.8% with the RSDs less 
      than 10.7%. Finally, the established MSPE-GC-MS method was successfully applied 
      to determine PMEs in human urine samples. MMP, MEP, MBP and MEHP were detected in 
      most of the samples with the median concentration of 8.46, 9.26, 13.60, and 5.95 
      ng mL(-1) respectively. MBzP was detected in 58.3% of the samples with the median 
      concentration of 3.05 ng mL(-1). Taken together, the MSPE-GC-MS method developed 
      in current study provides a new option for the determination of PMEs in human 
      urine.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Rastkari, Noushin
AU  - Rastkari N
AD  - Center for Air Pollution Research (CAPR), Institute for Environmental Research 
      (IER), Tehran University of Medical Sciences, Tehran 1417614411, Iran. 
      nr_rastkari@yahoo.com
FAU - Ahmadkhaniha, Reza
AU  - Ahmadkhaniha R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130227
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Biomarkers)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Phthalic Acids)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Biomarkers/urine
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Limit of Detection
MH  - Magnetite Nanoparticles/*chemistry
MH  - Male
MH  - Nanotubes, Carbon/*chemistry
MH  - Phthalic Acids/*isolation & purification/*urine
MH  - Reproducibility of Results
MH  - Sodium Chloride/chemistry
MH  - Solid Phase Extraction/*methods
MH  - Young Adult
EDAT- 2013/03/21 06:00
MHDA- 2013/08/27 06:00
CRDT- 2013/03/21 06:00
PHST- 2012/11/27 00:00 [received]
PHST- 2013/02/20 00:00 [revised]
PHST- 2013/02/21 00:00 [accepted]
PHST- 2013/03/21 06:00 [entrez]
PHST- 2013/03/21 06:00 [pubmed]
PHST- 2013/08/27 06:00 [medline]
AID - S0021-9673(13)00377-4 [pii]
AID - 10.1016/j.chroma.2013.02.070 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Apr 19;1286:22-8. doi: 10.1016/j.chroma.2013.02.070. Epub 
      2013 Feb 27.