PMID- 23512074
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20240109
IS  - 1537-4505 (Electronic)
IS  - 1531-7129 (Linking)
VI  - 34
IP  - 6
DP  - 2013 Aug
TI  - UHV-alginate as matrix for neurotrophic factor producing cells--a novel 
      biomaterial for cochlear implant optimization to preserve inner ear neurons from 
      degeneration.
PG  - 1127-33
LID - 10.1097/MAO.0b013e3182804949 [doi]
AB  - HYPOTHESIS: Ultra high viscous (UHV-) alginate is a suitable matrix for 
      brain-derived neurotrophic factor (BDNF) producing cells, enabling cell survival 
      and BDNF release out of the matrix and subsequent protection of auditory neuronal 
      cells. BACKGROUND: Cochlear implant (CI) target cells, spiral ganglion cells 
      (SGC), undergo a progressive degeneration. BDNF prevents SGC from degeneration 
      but has to be delivered locally to the inner ear for months. A permanent growth 
      factor application may be realized via a cell-based drug delivery system. 
      Encapsulation of this delivery system into a matrix could avoid immune response 
      of the recipient, migration, and uncontrolled proliferation of the cells. 
      METHODS: NIH3T3-fibroblasts producing endogenous BDNF were incorporated in 
      UHV-alginate. The survival of the cells in the alginate was examined by cell 
      counts of cryogenic slices, and the BDNF production was determined by performing 
      ELISA. The supernatant of the alginate-cell culture was added to primary SGC 
      culture, and the neuroprotective effect of the produced BDNF was observed 
      performing SGC counts. RESULTS: BDNF-producing cells cultivated in UHV-alginate 
      survived for up to 30 days, which was the latest time point observed. The BDNF 
      concentration in cell culture medium, produced from in UHV-alginate incorporated 
      fibroblasts and released out of the alginate matrix into the medium, was 
      significantly increased after 30 days of cultivation. Supernatant of 7 days 
      incubated UHV-alginate containing NIH3T3/BDNF cells significantly increased the 
      SGC survival in vitro. CONCLUSION: This study demonstrates UHV-alginate to be a 
      suitable scaffold for BDNF-producing fibroblasts. UHV-alginates are a promising 
      biomaterial for cochlear implant biofunctionalization.
FAU - Hutten, Mareike
AU  - Hutten M
AD  - Department of Otolaryngology, Head and Neck Surgery, Hannover Medical School, 
      Hannover, Germany.
FAU - Ehrhart, Friederike
AU  - Ehrhart F
FAU - Zimmermann, Heiko
AU  - Zimmermann H
FAU - Reich, Uta
AU  - Reich U
FAU - Esser, Karl-Heinz
AU  - Esser KH
FAU - Lenarz, Thomas
AU  - Lenarz T
FAU - Scheper, Verena
AU  - Scheper V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Otol Neurotol
JT  - Otology & neurotology : official publication of the American Otological Society, 
      American Neurotology Society [and] European Academy of Otology and Neurotology
JID - 100961504
RN  - 0 (Alginates)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Nerve Growth Factors)
SB  - IM
EIN - Otol Neurotol. 2013 Dec;34(9):1751. Erhacrt, Friederike [corrected to Ehrhart, 
      Friederike]
MH  - Alginates/chemistry/isolation & purification/*therapeutic use
MH  - Animals
MH  - Animals, Newborn
MH  - *Biocompatible Materials
MH  - Cell Count
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Ear, Inner/*innervation/*metabolism
MH  - Fibroblasts/metabolism
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Nerve Degeneration/*prevention & control
MH  - Nerve Growth Factors/*biosynthesis
MH  - Neurons/*metabolism
MH  - Phaeophyceae/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spiral Ganglion/cytology
MH  - Viscosity
EDAT- 2013/03/21 06:00
MHDA- 2014/02/14 06:00
CRDT- 2013/03/21 06:00
PHST- 2013/03/21 06:00 [entrez]
PHST- 2013/03/21 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
AID - 10.1097/MAO.0b013e3182804949 [doi]
PST - ppublish
SO  - Otol Neurotol. 2013 Aug;34(6):1127-33. doi: 10.1097/MAO.0b013e3182804949.